光棘球海胆体腔液调理素样分子的研究  

Opsonin-Like Molecule from Coelomic Fluid of Sea Urchin Strongylocentrotus nudus

作　　者：娄越 陈丹 常亚青[1] 丛玉婷[1] 王连顺[1] 李丹彤[1] LOU Yue;CHEN Dan;CHANG Yaqing;CONG Yuting;WANG Lianshun;LI Dantong(Laboratory of Mariculture & Stock Enhancement in North China′s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学,农业农村部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《水产科学》2021年第3期422-427,共6页Fisheries Science

基　　金：大连海洋大学农业农村部北方海水增养殖重点实验室基金资助项目(2018KF10).

摘　　要：为研究光棘球海胆的体液免疫机理,首先测定在不同反应基质中等量光棘球海胆吞噬细胞对不同处理的酵母细胞的吞噬率,其次比较分析从受调理酵母细胞和非调理酵母细胞以及光棘球海胆无细胞体腔液分离出成分的样品的电泳结果,随后将光棘球海胆无细胞体腔液进行提取分离后得到调理素样分子,最后于等渗缓冲溶液中测定等量光棘球海胆吞噬细胞在不同浓度调理素样分子和不同时间点作用下的吞噬率的变化。试验结果显示,光棘球海胆吞噬细胞分别在等渗缓冲液、经酵母细胞吸附过无细胞体腔液、光棘球海胆无细胞体腔液对非调理的酵母细胞和在等渗缓冲液中对受调理的酵母细胞的吞噬率之比为1.00∶1.17∶2.00∶1.71。在3个样品里,仅有受调理酵母细胞分离出来的成分的电泳结果显示为1个明显条带。等渗缓冲溶液里,分别加入0.5、1.0、1.5 mL经纯化的调理素样分子后,4个时间点吞噬率均高于对照组,当调理素样分子浓度升高,吞噬率亦增加。试验结果表明,在光棘球海胆体腔液里含有某种调理素样分子,分子质量约156 ku,能促进光棘球海胆体腔液中吞噬细胞的吞噬作用。In order to study the immune mechanism of coelomic fluid of sea urchin Strongylocentrotus nudus,we researched the phagocytosis activity of phagocytes in different media and different treatments of yeast cell.The composition isolated from the non-cell coelomic fluid,regulating yeast cells and non-regulating yeast cells was analyzed with SDS-PAGE.We got the isolated opsonin-like molecule from the non-cell coelomic fluid of the sea urchin after dialysed.We determined the phagocytosis in isotonic buffer affected by opsonin-like molecule of different concentration and different time.Results showed that the phagocytosis rate of non-regulating yeast cells in isotonic buffer,cell-free coelomic fluid by yeast cell adsorption,non-cell coelomic fluid and regulating yeast cells in isotonic buffer was 1.00∶1.17∶2.00∶1.71.Only the composition isolated from the regulating yeast cells after SDS-PAGE had a distinct band in three different compositions.Added 0.5 mL,1.0 mL,and 1.5 mL purified opsonin-like molecule in isotonic buffer,other groups were higher than the control group in each test time,and the greater the opsonin-like concentration,the higher the phagocytosis.These findings indicate that there is a kind of new opsonin-like molecule in coelomic fluid of sea urchin S.nudus with molecular weight of 156 ku which can promote phagocytosis.

关 键 词：光棘球海胆 吞噬活性 调理素样分子 吞噬细胞 

分 类 号：S968.9[农业科学—水产养殖]