不同碳源调控辅酶Ⅰ对聚磷菌胞内多聚物代谢的影响  

Effects of Coenzyme Ⅰ on Intracellular Polymer Metabolism of Phosphate Accumulating Organisms

作　　者：李丽 张华 张文超 LI Li;ZHANG Hua;ZHANG Wenchao(School of Environment and Energy Engineering,Anhui Jianzhu University,Anhui Hefei,230601,China;Key Laboratory of Anhui Province of Water Pollution Control and Wastewater Resuse,Anhui Hefei,230601,China)

机构地区：[1]安徽建筑大学环境与能源工程学院,安徽合肥230601 [2]水污染控制与废水资源化安徽省重点实验室,安徽合肥230601

出　　处：《安徽建筑大学学报》2021年第1期68-72,共5页Journal of Anhui Jianzhu University

基　　金：安徽省自然科学研究面上项目(1808085MB34);安徽省高校省级自然科学研究项目重大项目(KJ2019ZD52);安徽建筑大学引进人才及博士启动基金项目(2018QD20)。

摘　　要：辅酶Ⅰ是聚磷菌胞内物质代谢的关键酶,研究不同碳源调控辅酶Ⅰ对聚磷菌胞内PHB、poly-P、糖原代谢的影响对强化生物除磷具有重要的意义。研究结果表明,以乙酸钠为碳源对胞内辅酶Ⅰ进行调控时,胞内NAD+(氧化型辅酶Ⅰ)、NADH(还原型辅酶Ⅰ)平均含量分别为3.547μmol/g、3.255μmol/g,以葡萄糖为碳源对胞内辅酶Ⅰ进行调控时,胞内NAD+、NADH平均含量分别为1.592μmol/g、1.495μmol/g,说明以乙酸钠为碳源对胞内辅酶Ⅰ的调控效果更好。不同碳源调控胞内辅酶Ⅰ对聚磷菌胞内多聚物代谢影响的研究结果表明,乙酸钠为碳源进行调控辅酶Ⅰ时,胞内PHB和poly-P的合成量更高,聚磷菌除磷效率更好,因此,以乙酸钠为碳源调控聚磷菌胞内辅酶Ⅰ时有助于提高胞内多聚物的代谢能力。说明通过不同碳源调控聚磷菌胞内NADH的含量可以提高生物除磷的效果,为强化生物除磷效率提供一种新思想。During the biological phosphorus removal,coenzymeⅠis the key enzyme for the intracellular polymer metabolism of phosphate accumulating organisms.Therefore,it is important to study the effects of coenzyme on the metabolism of PHB,poly-P and glycogen of phosphate accumulating organisms.In this study,different carbon were used to regulate the coenzyme of the phosphate accumulating organisms,and then the metabolism of the intracellular polymers was further studied.The results showed that,when sodium acetate was used as the carbon of the phosphate accumulating organisms,the average contents of NAD+(oxidized coenzymeⅠ)and NADH(reduced coenzymeⅠ)were respectively 3.547μmol/g and 3.255μmol/g.However,when glucose was used as the carbon of the phosphate accumulating organisms,the average contents of NAD+and NADH were 1.592μmol/g and 1.495μmol/g respectively.The research indicated that,when sodium acetate was used as the carbon of the phosphate accumulating organisms,there was a better regulated effect on coenzymeⅠ.The metabolisms of the intracellular polymers indicated that,when sodium acetate was used as the carbon to regulate coenzymeⅠ,the synthesis of the intracellular PHB and poly-P were higher.The higher the synthesis of the intracellular PHB and poly-P were,the better the phosphorus removal efficiency was.Therefore,when sodium acetate was used as carbon to regulate the intracellular coenzymeⅠ,it was helpful to improve the metabolic capacity of intracellular polymers.The results showed that the biological phosphorus removal may be improved by regulating the intracellular coenzymeⅠof phosphate accumulating organism.This study provides a new idea for enhancing biological phosphorus removal.

关 键 词：碳源 辅酶Ⅰ PHB POLY-P 糖原 

分 类 号：X703.1[环境科学与工程—环境工程]