不育男性精子自发顶体反应率、顶体酶活性与精液常规参数及精子功能指标的相关性分析  

Correlation of sperm spontaneous acrosome reaction rate and acrosomal enzyme activity with semen routine parameters and sperm function indexes in infertile men

作　　者：周欢群[1] 蒋满波[1] 周文[1] 谢妍[1] 吴爱华[1] 王爱爱[1] 倪运萍[1] 陆杉[1] ZHOU Huanqun;JIANG Manbo;ZHOU Wen;XIEYan;WU Aihua;WANG Ai'ai;NI Yunping;LU Shan(Department of Reproductive Medicine,Traditional ChineseMedicine Hospital of Guangdong Province,GuangZhou 510006,Guangdong,China)

机构地区：[1]广东省中医院生殖医学科,广州510006

出　　处：《中国性科学》2021年第4期10-13,共4页Chinese Journal of Human Sexuality

基　　金：广东省卫生和计划生育委员会基金(A2017152)。

摘　　要：目的探讨不育男性精子自发顶体反应率、顶体酶活性与精液常规参数及精子功能指标的相关性。方法选取2019年4月至2020年2月广东省中医院生殖医学科诊治的77例不育男性作为研究对象。收集其实验室检测的精液分析数据进行回顾性分析。收集患者精子自发顶体反应、顶体酶活性、精子-透明质酸结合试验、精子DNA碎片、精子核蛋白、混合抗球蛋白反应试验(MAR)、精液常规分析的数据资料。采用Spearman相关性分析精子自发顶体反应率、顶体酶活性与精液常规参数及精子功能指标的相关性。结果精子自发顶体反应率与精子总数、尾部畸形率呈正相关(r=0.221、0.278,P=0.045、0.015),与平均路径速度(VAP)、顶体酶活性呈负相关(r=-0.223、-0.253,P=0.043、0.037);精子顶体酶活性与精子存活率呈正相关(r=2.84,P=0.018),与尾部畸形率呈显著负相关(r=-0.304,P=0.001)。精子自发顶体反应率、顶体酶活性与精子DNA碎片率、精子核蛋白不成熟度、精子-透明质酸结合率及MAR均无明显相关性(P>0.05)。结论精子自发顶体反应率、顶体酶活性与部分精液参数有相关性,但与多项精子功能指标无明显相关性。Objective To investigate the correlation among sperm spontaneous acrosome reaction rate and acrosomal enzyme activity with semen routine parameters and sperm function indexes in infertile men.Methods 77 infertile men treated in the Traditional Chinese Medicine Hospital of Guangdong Province from April 2019 to February 2020 were selected as the research subjects.Semen analysis data from laboratory tests were collected for retrospective analysis.The data of sperm spontaneous acrosome reaction,acrosomal enzyme activity,sperm-hyaluronic acid binding test,sperm DNA fragment,sperm nucleoprotein,mixed antiglobulin reaction test(MAR),and routine analysis of semen were collected.Spearman correlation was used to analyze the correlation of spontaneous acrosome reaction rate and acrosomal enzyme activity with semen routine parameters and sperm function indexes.Results The spontaneous acrosome reaction rate of sperm was positively correlated with the total number of sperm and the rate of tail malformation(r=0.221,0.278,P=0.045,0.015),and negatively correlated with the average path velocity(VAP)and acrosomal enzyme activity(r=-0.223,-0.253,P=0.043,0.037).The sperm acrosomal enzyme activity was positively correlated with the sperm survival rate(r=2.84,P=0.018),and negatively correlated with the rate of tail malformation(r=-0.304,P=0.001).Sperm spontaneous acrosomal reaction rate and acrosomal enzyme activity had no significant correlation with sperm DNA fragmentation rate,sperm nucleoprotein immaturity,sperm-hyaluronic acid binding rate and MAR(P>0.05).Conclusions Sperm spontaneous acrosome reaction,acrosin activity were correlated with some semen parameters,but has no significant correlation with multiple sperm function indexes.

关 键 词：自发顶体反应 顶体酶活性 精子DNA碎片 精子核蛋白 精子-透明质酸结合试验 

分 类 号：R697[医药卫生—泌尿科学]