阴道微环境相关因素对水通道蛋白2mRNA在阴道上皮细胞中表达的影响  

Effects of vaginal microenvironment related factors on AQP2mRNA expression in vaginal epithelial cells

作　　者：张晶 周自云[2] 夏海萍 马洁桦[4] ZHANG Jing;ZHOU Ziyun;XIA Haiping;MA Jiehua(Department of Pharmacy,Jiangsu Health Vocational College,Nanjing 210000,Jiangsu,China;Department of Child Healthcare Department,Wuxi Children's Hospital,Wuxi 214000,Jiangsu,China;Department of Clinical laboratory,Rizhao People's Hospital,Rizhao 276800,Shandong,China;Department of Medical Research Center,Nanjing Maternal and Child Health College,Narnjing 210000,Jiangsu,China)

机构地区：[1]江苏卫生健康职业学院药学院,南京210000 [2]无锡市儿童医院儿童保健科,江苏无锡214000 [3]日照市人民医院检验科,山东日照276800 [4]南京市妇幼保健院医学研究中心,南京210000

出　　处：《中国性科学》2021年第4期32-35,共4页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金(81501239,81771572);江苏省高等学校自然科学研究面上项目(18KJB320002);2019江苏高校“青蓝工程”资助;江苏卫生健康职业学院校级科研项目(JKB201912)。

摘　　要：目的明确阴道微环境对水通道蛋白2(AQP2)mRNA在阴道上皮细胞中表达的影响。方法选取2014年5月购自美国菌种保藏中心(ATCC)的VK2/E6E7细胞作为研究对象。体外培养VK2/E6E7细胞,分别予以不同浓度的雌二醇、睾酮、孕酮、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)及不同pH的培养基干预细胞,观察并收集干预24h及48h后的细胞,采用Real Time PCR技术,明确AQP2mRNA的表达水平。结果高浓度雌二醇可增加AQP2mRNA表达水平;较低浓度孕酮增加AQP2mRNA表达水平,高浓度孕酮降低AQP2mRNA表达水平;睾酮、炎症因子(IL-6、IL-8)及pH均对AQP2mRNA表达水平无显著影响规律,但不同pH会影响阴道上皮细胞的生存状态,进而影响阴道润滑。结论阴道微环境会影响AQP2mRNA在阴道上皮细胞中的表达,雌激素缺乏可能是女性阴道润滑障碍的致病因素。Objective To investigate the effect of vaginal microenvironment on AQP2 mRNA expression in vaginal epithelial cells. Methods The VK2/E6 E7 cells purchased from American type culture collection(ATCC) in May 2014 were selected as the research objects.VK2/E6 E7 cells were cultured in vitro. The cells were pretreated with different concentrations of estradiol, testosterone, progesterone, IL-6, IL-8 and different pH values. The cells were observed and collected 24 hours and 48 hours after intervention. The expression of AQP2 mRNA was determined by real time PCR. Results High concentration of estradiol can increase the expression level of AQP2 mRNA. Low concentration of progesterone increased the expression level of AQP2 mRNA, while high concentration of progesterone decreased the expression level of AQP2 mRNA. Testosterone, inflammatory cytokines(IL-6, IL-8) and pH had no significant effect on the expression level of AQP2 mRNA, but different pH would affect the survival state of vaginal epithelial cells, and then affect vaginal lubrication. Conclusions Vaginal microenvironment can affect the expression of AQP2 mRNA in vaginal epithelial cells. Estrogen deficiency may be the pathogenic factor of vaginal lubrication disorders in women.

关 键 词：阴道微环境 女性性功能障碍 水通道蛋白2mRNA 

分 类 号：R711[医药卫生—妇产科学]