自拟固胎煎对复发性流产小鼠外周血IL-6、IL-10、IL-17及TGF-β1的影响  

Effects of self-prepared Gutai Decoction on IL-6,IL-10,IL-17 and TGF-β1 in peripheral blood of mice with recurrent abortion

作　　者：周兰 徐彩[2] 黄海涛[2] 左茜茜 张芸娜[2] 黄文玲[2] 卜晓玲[2] 陈倩 ZHOU Lan;XU Cai;HUANG Haitao;ZUO Qianqian;ZHANG Yunna;HUANG Wenling;BU Xiaoling;CHEN Qian(The Sacond Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100029,China;Department of Gynecology,Beijing University of Chinese Medicine Dongfung Hospital,Beijing 100078,China;College of Integrated Traditional Chinese and Western Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050091,Hebei,Chin)

机构地区：[1]北京中医药大学第二临床医学院,北京100029 [2]北京中医药大学东方医院妇科,北京100078 [3]河北中医学院中西医结合学院,石家庄050091

出　　处：《中国性科学》2021年第4期134-137,共4页Chinese Journal of Human Sexuality

基　　金：2018年度校级青年教师项目(2018-JYBZZ-JS105)。

摘　　要：目的观察自拟固胎煎对复发性流产模型小鼠外周血白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)、转化生长因子-β1(TGF-β1)表达及对妊娠结局的影响。方法选取SPF级CBA/J雌鼠(n=48)、BALB/C雄鼠(n=4)、DBA/2雄鼠(n=20)作为研究对象。采用CBA/J雌鼠×BALB/c雄鼠建立正常妊娠模型,以CBA/J雌鼠×DBA/2雄鼠建立复发性流产模型,复发性流产模型孕鼠随机分为中药组、地屈孕酮组和复发性流产组,且将中药组分为高剂量亚组、中剂量亚组、低剂量亚组,正常妊娠组和复发性流产组给予生理盐水,中药高剂量亚组、中剂量亚组、低剂量亚组依次给予28.5g/kg、14.25g/kg、7.125g/kg自拟固胎煎汤剂,地屈孕酮组给予3.0mg/kg地屈孕酮溶液,连续灌胃2周,每天1次。给药结束后处死并解剖小鼠,观察子宫胚胎数量并计算胚胎丢失率,酶联免疫吸附试验(ELISA)法检测外周血IL-6、IL-10、IL-17及TGF-β1的表达水平。结果与复发性流产组比较,中药中剂量亚组、高剂量亚组和地屈孕酮组胚胎丢失率均显著降低,差异具有统计学意义(P<0.05);与复发性流产组比较,中药中剂量亚组、高剂量亚组和地屈孕酮组的IL-6和IL-17的表达均显著降低,IL-10和TGF-β1的表达均显著升高,差异具有统计学意义(P<0.05)。结论自拟固胎煎可能通过上调IL-10、TGF-β1的表达水平,下调IL-6、IL-17的表达水平,诱导免疫耐受,减少胚胎丢失率,从而改善妊娠结局。Objective To observe the effects of self-prepared Gutai Decoction on the expression of interleukin-6(IL-6),interleukin-10(IL-10),interleukin-17(IL-17),transforming growth factor-β1(TGF-β1)in peripheral blood of recurrent abortion model mice and the pregnancy outcome.Methods SPF CBA/J female rats(n=48),BALB/C male rats(n=4)and DBA/2 male rats(n=20)were selected as the study subjects.CBA/J female mouse and BALB/c male mouse were made as the normal pregnancy group,and CBA/J female mouse and DBA/2 male mouse as the recurrent spontaneous abortion group.The recurrent spontaneous abortion group was randomly divided into Chinese medicine group,dydrogesterone group,and recurrent abortion group.And Chinese medicine group was divided into high,medium and low dose subgroup.Normal pregnancy group and recurrent spontaneous abortion group were given saline.High,medium and low dose subgroups in Chinese medicine group were given 28.5 g/kg、14.25 g/kg、7.125 g/kg self-prepared Gutai Decoction,and the dydrogesterone group was given 3.0 mg/kg dydrogester,all with stomach perfusion for 2 weeks,once a day.After that,mice were dissected to observe the number of uterine embryos and calculate the rate of embryo loss,and detect the expression levels of IL-6,IL-10,IL-17 and TGF-β1 in peripheral blood by enzyme-linked immunosorbent assay(ELISA).Results Compared with recurrent abortion group,embryo loss rate in medium dose subgroup,high dose subgroup and didrogesterone group was significantly decreased(P<0.05).The expressions of IL-6 and IL-17 in medium dose subgroup,high dose subgroup and didrogesterone group were significantly decreased,and the expressions of IL-10 and TGF-β1 were significantly increased(P<0.05).Conclusions Self-prepared Gutai Decoction may improve the pregnancy outcome by up-regulating the expression levels of IL-10 and TGF-β1,down-regulating the expression levels of IL-6 and IL-17,inducing immune tolerance and reducing the rate of embryo loss.

关 键 词：中医药 白细胞介素-6 白细胞介素-10 白细胞介素-17 转化生长因子-Β1 复发性流产 

分 类 号：R275[医药卫生—中医皮科]