正交试验法优选浙江金线兰多糖提取工艺研究  

Optimization of Extraction Technology of Polysaccharides from Anoectochilus zhejiangensis by Orthogpnal Method

作　　者：江颖倩 彭梦超 吴岩斌[1] 吴锦忠[1] JIANG Ying-qian;PENG Meng-chao;WU Yan-bin;WU Jin-zhong(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《海峡药学》2021年第4期31-34,共4页Strait Pharmaceutical Journal

基　　金：福建省卫生健康科技计划项目资助(2019-ZQNB-11);国家自然科学基金资助项目(81773846,81603230)。

摘　　要：目的正交试验法优选浙江金线兰多糖最佳提取工艺。方法以料液比、提取时间、提取温度、提取次数为考察因素,采用苯酚-硫酸法测定吸光度值,以多糖提取含量为评价指标,采用L 9(34)正交试验法优选浙江金线兰多糖提取工艺。结果影响浙江金线兰多糖提取含量主次顺序为C提取温度>D提取次数>A料液比>B提取时间,提取的最佳工艺为料液比1∶30,提取时间120 min,提取温度100℃,提取次数4次,提取含量为9.95±0.23%。结论该提取工艺稳定可行,重现性好,为浙江金线兰多糖提取的最佳工艺。OBJECTIVE To optimize the best extraction process of polysaccharides from Anoectochilus zhejiangensis by orthogonal method.METHODS The ratio of material to water,extraction times,extraction temperature and extraction time as factors and absorbance value was determined by phenol-sulfuric acid method.The L 9(34)orthogonal test was used to optimize the extraction technology of polysaccharides from A.zhejiangensis.RESULTS The effect of extraction yield in descending order was extraction temperature>extraction times>ratio of material to wate>extraction time.The optimal extraction conditions polysaccharides from A.zhejiangensis were as follows:ratio of material to rate 1∶30,extraction time 120 min,extraction temperature 100℃and extraction repeated 4 times,the extraction yield was(9.95±0.23)%.CONCLUSION The optimal extraction process was stable and reproducible,which suitable for the extraction of polysaccharides from A.zhejiangensis.

关 键 词：浙江金线兰 多糖 提取工艺 正交设计试验 

分 类 号：R284[医药卫生—中药学]