参茯消甘饮HPLC指纹图谱的研究  

HPLC Fingerprint of the ShenFu XiaoGan Drink

作　　者：朱萍 李国维 秦昆明 杨超[4] 孙戡平 蔡宝昌 金俊杰[2,3] ZHU Ping;LI Guo-wei;QIN Kun-ming;YANG Chao;SUN Kan-ping;CAI Bao-chang;JIN Jun-jie(The First People′s Hospital of Zhangjiagang,Zhangjiagang 215600,China;Nanjing Haichang Chinese Medicine Corporation,Nanjing 210061,China;Nanjing Haiyuan Prepared Slices of Chinese Crude Drugs Co.Ltd,Nanjing 210061,China;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]张家港市第一人民医院,江苏张家港215600 [2]南京海昌中药集团有限公司,江苏南京210061 [3]南京海源中药饮片有限公司,江苏南京210061 [4]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023

出　　处：《海峡药学》2021年第4期59-61,共3页Strait Pharmaceutical Journal

基　　金：“重大新药创制”科技重大专项(2017ZX09301075);江苏省科技成果转化专项资金项目(BA2018002);南京市科技计划项目(201812021)。

摘　　要：目的建立参茯消甘饮的HPLC指纹图谱,为其质量评价提供理论依据。方法采用RP-HPLC法。色谱柱为YMC-Pack ODS-A(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸水溶液梯度洗脱,体积流量:0.9 mL·min^(-1),柱温:40℃,检测波长:254 nm。用国家药典委员会编“相似度评价软件(2004A)”处理分析,建立参茯消甘饮的HPLC指纹图谱。结果确立了16个共有峰的共有模式,8个批次参茯消甘饮样品指纹图谱各色谱峰的分离较好,符合指纹图谱检测要求。结论本研究所建立的方法可靠、简便易行,为参茯消甘饮的质量控制提供了科学依据。OBJECTIVE To establish HPLC fingerprint of ShenFu XiaoGan drink,and provide a theoretical basis for its quality evaluation.METHODS RP-HPLC method was used.Column chromatography YMC-Pack ODS-A(250 mm×4.6 mm,5μm),acetonitrile-0.1%phosphoric acid solution gradient elution,flow volume:0.9 mL·min^(-1),column temperature:40℃,and the detection wavelength:254 nm.With the State Pharmacopoeia Commission“similarity evaluation software(2004a)”processing and analysis,to establish ShenFu XiaoGan drink HPLC fingerprint.RESULTS 16 common peaks were established,8 batches of ShenFu XiaoGan drink sample fingerprint chromatography of the peak was better separated,in line with the fingerprint.CONCLUSION This method is simple,reliable,and it provide a scientific basis for the quality control of ShenFu XiaoGan drink.

关 键 词：参茯消甘饮 指纹图谱 HPLC 葛根素 甘草素 

分 类 号：R927[医药卫生—药学]