耐钼细菌选育驯化技术研究  

Domestication of Bacteria to Tolerate Molybdenum

作　　者：程浩[1] 孟运生[1] 刘辉[1] 周磊[1] 师留印[1] 张静敏[1] CHENG Hao;MENG Yunsheng;LIU Hui;ZHOU Lei;SHI Liuyin;ZHANG Jingmin(Beijing Research Institute of Chemical Engineering and Metallurgy,CNNC,Beijing 101149,China)

机构地区：[1]核工业北京化工冶金研究院,北京101149

出　　处：《铀矿冶》2021年第2期128-133,共6页Uranium Mining and Metallurgy

摘　　要：为解决细菌浸出辉钼矿过程中钼对细菌的抑制问题,提高细菌对钼的耐受能力,对三株细菌进行耐钼驯化研究,结果显示氧化亚铁硫杆菌、氧化亚铁钩端螺旋菌、野生菌株LM1的耐钼质量浓度分别达到820 mg/L、620 mg/L和580 mg/L。对野生菌株LM1进行的生理生化特性研究和分子生物学鉴定表明,LM1菌株属于嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)。In order to solve the problem of inhibition of molybdenum on bacteria in the process of bioleaching molybdenite and improving the tolerance of bacteria to molybdenum,three strains of bacteria were acclimated to molybdenum.The results show that,the molybdenum tolerance of Thiobacillus ferrooxidans,Leptospirillum ferrooxidans and the wild strain LM1 were 820 mg/L,620 mg/L and 580 mg/L,respectively.The morphological,biochemical phsiological characters of bacterial bacterial strain LM1 were studied.LM1 is a new strain of Acidithiobacillus ferrooxidans.

关 键 词：驯化 分子生物学鉴定 生理生化特性 耐钼细菌 

分 类 号：TF841.2[冶金工程—有色金属冶金]