水稻响应冷胁迫基因OsHI-XIP过量表达载体构建及遗传转化体系建立  

Construction of Over-expression Vector and Genetic Transformation System of OsHI-XIP Gene Responding to Cold Stress in Rice

作　　者：熊文涛 沈雨民[1] 陈明亮[1] 罗世友[1] 熊焕金[1] 吴小燕[1] 肖叶青[1] XIONG Wen-tao;SHEN Yu-min;CHEN Ming-liang;LUO Shi-you;XIONG Huan-jin;WU Xiao-yan;XIAO Ye-qing(Rice Research Institute,Jiangxi Academy of Agricultural Sciences/National Engineering Laboratory for Rice(Nanchang)/Nanchang Sub-center,Nanchang Rice Improvement Center,Nanchang 330200,China)

机构地区：[1]江西省农业科学院水稻研究所/水稻国家工程实验室(南昌)/国家水稻改良中心南昌分中心,江西南昌330200

出　　处：《江西农业学报》2021年第5期42-45,62,共5页Acta Agriculturae Jiangxi

基　　金：国家自然科学地区基金项目“东乡野生稻耐冷候选基因的功能验证及分子机理研究”(31860367);江西省青年科学基金项目“东乡野生稻中耐冷基因的功能验证”(20171BAB214025)。

摘　　要：通过转录组比较分析筛选到6个水稻响应冷胁迫的基因,其中OsHI-XIP基因被重点研究。为了深入研究OsHI-XIP基因的生物学功能,从越光品种基因组中克隆了这个基因的序列,并构建了该基因的过量表达载体。通过农杆菌介导法将该载体导入赣香B株系的胚性愈伤组织中,获得了转基因阳性苗16株。本研究首次建成了赣香B的遗传转化体系,转化效率高达16.4%,明显高于一般籼稻品种的转化效率,为籼稻遗传转化及育种研究提供了技术支撑。Six rice genes responding to cold stress were screened through transcriptome comparative analysis,among which the OsHI-XIP gene was focused on.In order to further study the biological function of this gene,the OsHI-XIP gene sequence was cloned from the genome of Koshihikari variety,and an over-expression vector of the gene was constructed.The vector was introduced into the embryogenic callus of Ganxiang B strain by Agrobacterium-mediated method,and 16 transgenic positive seedlings were obtained.In the study,the genetic transformation system of Ganxiang B was established for the first time.The transformation efficiency was as high as 16.4%,which was significantly higher than that of general indica rice varieties.It provided technical support for indica rice genetic transformation and breeding research.

关 键 词：水稻 赣香B 东乡野生稻 低温 遗传转化 农杆菌介导法 

分 类 号：S511.034[农业科学—作物学]