巴戟天-续断-益母草组药干预肾虚血瘀型多囊卵巢综合征网络药理学作用机制  

Network Pharmacological Mechanism ofBajitian-Xuduan-Yimucao Drug Combination in the Internvetion for PCOS of Kidney-deficiency Blood-stasis Pattern

作　　者：袁仁智[1,2,3] 肖卫琼 唐鹏[1,2] 康开彪 潘文[2] YUAN Renzhi;XIAO Weiqiong;TANG Peng;KANG Kaibiao;PAN Wen(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Gansu Provincial Academy of Chinese Medicine;Shaoyang University)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃省中医药研究院 [3]邵阳学院

出　　处：《西部中医药》2021年第5期14-21,共8页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81660803)。

摘　　要：目的:运用网络药理学方法和技术研究“巴戟天-续断-益母草”组药治疗多囊卵巢综合征(polycystic ovarian syndrome,PCOS)的主要活性成分、靶点、通路等药理学作用机制。方法:通过数据挖掘获得PCOS肾虚血瘀型核心组药巴戟天-续断-益母草;检索中药系统药理学数据库分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP),筛选出巴戟天-续断-益母草的活性成分和潜在靶点;查询NCBI数据库获得PCOS疾病靶点,找到药物和疾病的共靶点;通过DAVID数据库对共靶点进行基因功能GO富集分析和KEGG富集分析;采用Cytoscape软件构建成分-靶标、靶标-通路网络。结果:从“巴戟天-续断-益母草”组药中筛选得到26个候选活性分子,作用于719个靶点,找到PCOS疾病靶点228个,两者共有靶点22个,主要涉及炎性反应、细胞因子、雌激素、胰岛素抵抗等生物学过程中的多条信号通路。结论:本研究初步揭示“巴戟天-续断-益母草”组药的主要活性成分、靶点及协同作用机制,为进一步深入探讨其药理学作用提供了参考。Objective:To study main active ingredients,target spot,pathway and others of"Bajitian-Xuduan-Yimucao"drug combinations in the treatment for polycystic ovarian syndrome(PCOS)using network pharmcological method and technology.Methods:Core drug combinations"Bajitian-Xuduan-Yimucao"for PCOS of kidney-deficiency blood-stasis pattern were obtained by data mining;active ingredients and potiential target spot of"Bajitian-Xuduan-Yimucao"were screened by searching traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP);PCOS disease target spots,common target spots of drugs and diseases were obtained by searching NCBI database;Gene function Go concentration analysis and KEGG concentration analysis of the common target spots were performed using DAVID database;ingredient-target spot,target spot-pathway network were constructed by adopting Cytoscape software.Results:All 26 candidate bioactive molecules were selected from"Bajitian-Xuduan-Yimucao"drug combinations,and they acted on 719 target spots,228 PCOS disease target spots were found,22 common target spots,and many signal channels about inflammtory reaction,cytokines,estrogen,insuslin resistance and others in the biological process were involved mainly.Conclusion:The study preliminarily reveals main active ingredients,target spots and synergistic mechanism of the drug combinations,and it could provide the reference for further exploration into its pharmacology.

关 键 词：多囊卵巢综合征 肾虚血瘀 网络药理学 巴戟天 续断 益母草 

分 类 号：R271[医药卫生—中医妇科学]