基因芯片技术分析HIV合并非结核分枝杆菌感染患者的菌种分布和免疫学特征  

Analysis of distribution and immunological characteristics of HIV co-infected with non-tuberculosis mycobacteria patients based on gene chip technology

作　　者：张桂仙 高丽 李正伦 张米 谢祺 ZHANG Guixian;GAO Li;LI Zhenglun;ZHANG Mi;XIE Qi(Department of Clinical Laboratory,Yunnan Provincial Hospital of Infectious Diseases,Kunming,Yunnan 650301,China)

机构地区：[1]云南省传染病医院检验科,云南昆明650301

出　　处：《国际检验医学杂志》2021年第10期1176-1179,共4页International Journal of Laboratory Medicine

基　　金：云南省高层次卫生健康技术人才培养专项经费资助项目(H-2018018);云南省教育厅科学研究基金项目(2020J0234、2018JS251)。

摘　　要：目的利用基因芯片技术检测非结核分枝杆菌(NTM),以此了解该院人类免疫缺陷病毒(HIV)合并NTM感染患者的菌种分布和免疫学特征。方法分析该院2012年9月至2019年12月从90例HIV感染者标本中分离到的90株NTM的分布情况,并分析患者的CD4+T淋巴细胞检测结果。结果90例HIV合并NTM感染患者中共检出鸟分枝杆菌73株(81.11%),堪萨斯分枝杆菌6株(6.67%),龟脓分枝杆菌4株(4.44%),戈登分枝杆菌2株(2.22%),瘰疬分枝杆菌2株(2.22%),偶然分枝杆菌2株(2.22%),浅黄分枝杆菌1株(1.11%)。CD4+T淋巴细胞<50个/μL的患者多为鸟分枝杆菌感染(42.22%);其次为堪萨斯分枝杆菌(4.44%),CD4+T淋巴细胞为50~200个/μL的患者感染鸟分枝杆菌也居多(28.89%)。结论鸟分枝杆菌是HIV合并NTM感染的主要菌种,基因芯片检测技术能够快速对NTM进行鉴定,并能用于多种类型标本检测,是值得推广的检测NTM感染的方法。Objective Non-tuberculosis mycobacteria(NTM)were detected by gene chip technology in order to understand the distribution and immunological characteristics of human immunodeficiency virus(HIV)co-infected with NTM in Yunnan Provincial Hospital of Infectious Diseases.Methods To analyze the distribution of 90 NTM strains isolated from HIV infected patients in Yunnan Hospital of Infectious Diseases from September 2012 to December 2019,and analyze the results of CD4+T lymphocytes.Results Among 90 HIV co-infected with NTM patients,73 strains of Mycobacterium Avium(81.11%),6 strains of M.Kansas(6.67%),4 strains of M.Chelonis(4.44%),2 strains of M.Gordon(2.22%),2 strains of Mycobacterium Scrofula(2.22%),2 strains of Mycobacterium Fortuitum(2.22%)and 1 strain of Mycobacterium Phlei(1.11%)were detected.The infection rate of Mycobacterium avium in patients with CD4+T lymphocyte less than 50 cells perμL was highest(42.22%),and followed with Kansas(4.44%),the CD4+T lymphocyte of Mycobacterium avium was also higher(28.89%)inpatients with CD4+T lymphocyte between 50 and 200 cells perμL.Conclusion Mycobacterium avium is the main bacteria of HIV co-infected with NTM,gene chip technology could rapidly identify NTM,and could be used for the detection of many types of specimens,which is worthy of promotion in the diagnosis and treatment of NTM infection.

关 键 词：非结核分枝杆菌 人类免疫缺陷病毒 CD4+T淋巴细胞 寡核苷酸序列分析 

分 类 号：R446.5[医药卫生—诊断学]