六星黑点豹蠹蛾性信息素活性成分的提取和初步鉴定  

The extraction and identification of active components of the sex pheromones of Zeuzera leuconotum Butler(Lepidoptera:Cossidae)

作　　者：刘红霞[1] 荆小院[2] 郑海霞[3] 杨美红[1] 张金桐[1] 朱卫华 刘金龙[1] LIU Hong-Xia;JING Xiao-Yuan;ZHENG Hai-Xia;YANG Mei-Hong;ZHANG Jin-Tong;ZHU Wei-Hua;LIU Jin-Long(Department of Basic Courses,Shanxi Agriculture University(Shanxi Academy of Agriculture Science),Taigu 030801,China;College of Life Science,Shanxi Agriculture University(Shanxi Academy of Agriculture Science),Taigu 030801,China;Colledge of Agriculture,College of Life Science,Shanxi Agriculture University(Shanxi Academy of Agriculture Science),Taigu 030801,China;Agricultural Technology Extension Service of Huairen,Huairen 035300,China)

机构地区：[1]山西农业大学(山西省农业科学院)基础部,太谷030801 [2]山西农业大学(山西省农业科学院)生命科学学院,太谷030801 [3]山西农业大学(山西省农业科学院)农学院,太谷030801 [4]山西省怀仁市农业技术推广中心,怀仁035300

出　　处：《应用昆虫学报》2021年第2期453-460,共8页Chinese Journal of Applied Entomology

基　　金：北京市科技计划“影响北京生态安全的重大钻蛀性害虫防控技术研究与示范”(Z171100001417005);国家重点研发计划“人工林重大灾害防控关键技术研究”(2018YFD0600200)。

摘　　要：【目的】初步鉴定六星黑点豹蠹蛾Zeuzera leuconotum Butler(Lepidoptera,Cossidae)雌蛾性信息素活性成分。【方法】采用正己烷溶剂浸提法提取六星黑点豹蠹蛾性信息素腺体中的化学组分,利用毛细管气相色谱(GC)和气相色谱-质谱联用(GC-MS)技术分析性腺提取物,并运用触角电位技术(EAG)测定提取物及标准化合物的电生理活性。【结果】GC结果显示六星黑点豹蠹蛾性腺体提取物中主要存在7种成分,其中G组分在加入标准化合物反-2-顺-13-十八碳烯醇-1-乙酸酯(E2,Z13-18:Ac)后,峰高和峰面积均相应增加。GC-MS分析结果证明G组分为E2,Z13-18:A。EAG结果显示7种组分均能引起雄蛾触角电位反应,其中对E2Z13-18:Ac的反应值最大。【结论】本研究初步鉴定出E2,Z13–18:Ac是六星黑点豹蠹蛾的性信息主要组分,为使用性信息素防治六星黑点豹蠹蛾奠定了基础。[Objectives] To identify the active components of the sex pheromone of female Zeuzera leuconotum Butler moths. [Methods] Sex pheromones were extracted from the sex glands of female moths with n-hexane and the active components analyzed using gas chromatography(GC) and gas chromatography–mass spectrometry(GC-MS). Electroantennograms(EAG) were used to examine the EAG responses of male antennae to extracts and standard components. [Results] GC results indicate that there are 7 chemicals in the extract, and that the peak height and area of component G increased after the addition of standard compound E2,Z13-18:Ac. Based on GC-MS data, component G was identified as E2,Z13-18:Ac. The EAG results show that all compounds caused an antennal reaction, and that E2,Z13-18:Ac elicited the strongest EAG activity. [Conclusion] E2,Z13-18:Ac was identified as the main component of the sex pheromone of Z. leuconotum. These results provide a theoretical basis for developing synthetic sex pheromone lures to control this pest.

关 键 词：六星黑点豹蠹蛾 性信息素 鉴定 触角电位 

分 类 号：S763.42[农业科学—森林保护学]