同卵、异卵、非双胞胎外周血TCRβ链假基因和功能性CDR3受体库前后5年动态变化分析  

Analysis for dynamic change of TCRβchain pseudogene and productive CDR3 repertoires in peripheral blood among monozygotic,dizygotic and non twins before and after 5 years

作　　者：苏丹华 董晓衡 贺晓燕 王小妹 马锐 马龙 姚新生(指导) SU Dan-Hua;DONG Xiao-Heng;HE Xiao-Yan;WANG Xiao-Mei;MA Rui;MA Long;YAO Xin-Sheng(Department of Immunology,Zunyi Medical University,Guizhou Key Laboratory of Genetic Testing and Characterization,Immunomolecular Engineering Research Center of Guizhou Province,Zunyi 563000,China)

机构地区：[1]遵义医科大学免疫教研室贵州省基因检测治疗与特色重点实验室,贵州省免疫分子工程研究中心,遵义563000

出　　处：《中国免疫学杂志》2021年第6期706-712,717,共8页Chinese Journal of Immunology

基　　金：国家自然科学基金(81860300);贵州省科学技术基金项目(黔科合LH字[2014]7568号);贵州省百层次人才基金(黔科合平台人才[2018]5637号)资助。

摘　　要：目的:初步探讨个体遗传因素对V(D)J重组过程中的偏向性影响和TCR的MHC-自身肽耐受的选择效应。方法:采用高通量测序技术分析同卵双胞胎(MZ)、异卵双胞胎(DZ)和非双胞胎(NT)外周血中TCRβ链假基因CDR3受体库与功能性CDR3受体库前后5年的动态变化。结果:TRBV基因取用,V-J配对,核苷酸的插入和删除前后5年的动态变化在MZ之间更为一致;而CDR3区TRBJ基因取用、AA取用、AA长度分布和Overlap前后5年的动态变化在MZ、DZ和NT之间无显著差异。结论:本实验提示遗传因素可能对外周血总T细胞CDR3受体库的偏向性产生影响。Objective:In order to preliminary explore the influence of individual genetic factors on bias of V(D)J recombina⁃tion and selection effect MHC-self peptide tolerance of TCR.Methods:High-throughput sequencing technology was used to analyze 5-year dynamic change of TCRβchain pseudogene and productive CDR3 repertoires among monozygotic twins(MZ),dizygotic twins(DZ),and non-twins(NT).Results:The 5-year dynamic change of TRBV gene usage,V-J pairing,and nucleotide insertion and dele⁃tion were more consistent between MZ;while the 5-year dynamic change of TRBJ gene usage,AA usage,AA length distribution and Overlap in CDR3 region did not differ significantly among MZ,DZ and NT.Conclusion:The experiment suggested that genetic factors may have a bias effect on the CDR3 repertoires of total T cells in peripheral blood.

关 键 词：同卵和异卵双胞胎 TCRβ链假基因CDR3受体库 高通量测序(HTS) 前后5年动态变化 

分 类 号：R392.9[医药卫生—免疫学]