人乳腺珠蛋白荧光免疫层析检测方法的建立  

Establishment of fluorescence immunochromatography for detection of human mammaglobin

作　　者：崔云会 陈楠[1,2] 王云龙(指导)[1,3] 明亮[1] 李玉林 王继创[1,3] CUI Yun-Hui;CHEN Nan;WANG Yun-Long;MING Liang;LI Yu-Lin;WANG Ji-Chuang(Laboratory Department of the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院检验科,郑州450052 [2]郑州人民医院,郑州450003 [3]河南省生物工程技术研究中心,河南450010

出　　处：《中国免疫学杂志》2021年第8期975-978,共4页Chinese Journal of Immunology

基　　金：河南省科技攻关项目(192102310418)。

摘　　要：目的:建立外周血中人乳腺珠蛋白(hMAM)荧光免疫层析快速定量检测方法。方法:采用双抗体夹心法制备免疫层析试纸条,并评价线性范围、精密性、特异性等性能指标。结果:实现了外周血中hMAM定量检测,线性范围0.312~20 ng/ml,批内变异系数均小于10%,批间变异系数均小于15%,平均回收率102.6%,与癌胚抗原(CEA)、癌抗原(CA153)无明显交叉反应,ELISA平行检测46例临床血清相关性良好。结论:建立了定量检测hMAM的时间分辨荧光免疫层析方法,为乳腺癌诊断和预后提供一种辅助检测手段。Objective:To establish a fluorescence immunochromatographic method for rapid and quantitative detection of human mammaglobin(hMAM)in peripheral blood.Methods:The double antibody sandwich method was used to prepare immunochromatographic test strips,and the performance indicators such as linear range,precision and specificity were evaluated.Results:The quantitative detection of hMAM in peripheral blood was realized.The linear range was 0.312~20 ng/ml.The intra-assay coefficient of variation was less than 10%,the inter-assay coefficient of variation was less than 15%,and the average recovery was 102.6%.There was no obvious cross-reaction with carcinoembryonic antigen(CEA)and cancer antigen(CA153).There was a good correlation with46 clinical serum detected in parallel with ELISA kit.Conclusion:A time-resolved fluorescence immunochromatographic method for quantitative detection of hMAM has been established,which provides an auxiliary detection method for the diagnosis and prognosis of breast cancer.

关 键 词：人乳腺珠蛋白 荧光免疫层析法 乳腺癌 

分 类 号：R446.61[医药卫生—诊断学]