TLR2、IL-10 mRNA的表达及树突状细胞分布在外阴硬化性苔藓中的临床意义  

Clinical significance of TLR2,IL-10 mRNA and dendritic cells in vulvar lichen sclerosus

作　　者：王楠楠[1] 王婧[1,2] 刘嗣同 武昕(指导) WANG Nan-Nan;WANG Jing;LIU Si-Tong;WU Xin(Department of Gynaecology,the First Affiliated Hospital of China Medical University,Shenyang 110000,China)

机构地区：[1]中国医科大学附属第一医院妇科,沈阳110000 [2]沈阳市妇婴医院妇科,沈阳110007

出　　处：《中国免疫学杂志》2021年第8期979-982,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30973190);辽宁省科学技术计划项目(2014021057)。

摘　　要：目的:探讨与免疫相关的TLR2、IL-10 mRNA及树突状细胞与外阴硬化性苔藓(VLS)发生和发展的关系。方法:选取VLS石蜡标本50例(早期和进展期各25例),外阴正常皮肤15例。采用免疫组化S-P法检测CD1α在组织中的分布,RT-PCR法检测TLR2和IL-10 mRNA在组织中的表达。结果:CD1α阳性细胞在VLS进展期中明显高于正常外阴皮肤(P<0.05)。TLR2、IL-10的mRNA表达在VLS进展期中明显高于正常外阴皮肤(P<0.05);TLR2、IL-10 mRNA和CD1α表达在早期VLS中略增加,与正常外阴皮肤相比无统计学差异(P>0.05)。结论:VLS的进展期TLR2、IL-10 mRNA及树突状细胞增多,预示局部组织内免疫抑制作用增强。Objective:To explore the clinical significance of TLR2、IL-10 mRNA and dendritic cell in vulvar lichen sclerosus(VLS).Methods:The CD1αpositive cells were examined with S-P immunohistochemistry,and the expression of TLR2 and IL-10 mRNA were detected through RT-PCR method in the lesions of 50 vulvar lichen sclerosus(25 early;25 advanced).The vulvar skins from 15 healthy individuals were selected as the control.Results:The CD1αpositive cells in advanced VLS was significantly higher than that in healthy vulvar skin(P<0.05).The expression of TLR2 and IL-10 mRNA in advanced VLS was significantly higher than that in healthy vulvar skin(P<0.05).The expressions of TLR2,IL-10 mRNA and CD1αwere slightly increased in early VLS,and there was no statistical difference compared with the normal vulva skin(P>0.05).Conclusion:The increased TLR2,IL-10 mRNA and dendritic cells in advanced VLS indicates the immunosuppression enhancement in local tissues.

关 键 词：外阴硬化性苔藓 TOLL样受体2 白介素-10 树突状细胞 CD1α 

分 类 号：R711[医药卫生—妇产科学]