外周血Th17与Treg细胞失衡与儿童EB病毒感染的相关性  

Correlation between the imbalance of peripheral blood Th17 cells and Treg cells and EB virus infection in children

作　　者：周洋[1] 陈芳芳 王桂兰[1] 齐曦明[2] 康厚鑫 吴小波[1] ZHOU Yang;CHEN Fangfang;WANG Guilan;QI Ximing;KANG Houxin;WU Xiaobo(The First Ward, Department of Pediatric, Qinhuangdao First Hospital, Qinhuangdao 066000, Hebei, China;Central Laboratory, Qinhuangdao First Hospital, Qinhuangdao 066000, Hebei, China)

机构地区：[1]秦皇岛市第一医院儿科一病区,河北秦皇岛066000 [2]秦皇岛市第一医院中心实验室,河北秦皇岛066000

出　　处：《西部医学》2021年第5期705-708,共4页Medical Journal of West China

基　　金：秦皇岛市重点研发计划科技支撑项目(201902A185)。

摘　　要：目的探讨外周血辅助性T细胞(Th17)与调节性T细胞(Treg)失衡与儿童EB病毒(EBV)感染的相关性。方法选取2019年12月~2020年5月秦皇岛市第一医院儿科收治的EBV感染患儿58例为EBV感染组,另选择同期本院健康体检儿童50例为对照组。采集空腹静脉血,检测并比较两组外周血Th17、Treg细胞表达率、RORγt mRNA、Foxp3 mRNA相对表达水平及细胞相关特征性细胞因子白介素17(IL-17)、转化生长因子-β1(TGF-β1)水平。结果EBV感染组患儿外周血Th17细胞表达率明显高于对照组,Treg细胞表达率和Treg/Th17细胞比率明显低于对照组(P<0.05);EBV感染组患儿RORγt mRNA表达率明显高于对照组,Foxp3 mRNA表达率明显低于对照组(P<0.05);EBV感染组患儿IL-17水平明显高于对照组,TGF-β1水平明显低于对照组(P<0.05)。结论EBV感染患儿的发病与Th17、Treg细胞失衡及其相关特征性细胞因子水平的变化密切相关。Objective To explore the correlation between the imbalance of peripheral blood h17 cells and Treg cells and Epstein-Barr virus(EBV)infection in children.Methods Fifty-eight children with EBV infection treated in the Pediatric Outpatient Department,The First Hospital of Qinhuangdao during the period from December 2019 to May 2020 were enrolled in the study.Meanwhile,50 healthy children were selected as the control group.Fasting venous blood was collected to detect the expression rates of peripheral blood Th17 cells and Treg cells,relative expression levels of RORγt mRNA and Foxp3 mRNA,and levels of cell-related characteristic cytokines IL-17 and TGF-β1.Results The expression rate of peripheral blood Th17 cells in EBV infection group was significantly higher than that in control group,and the expression rate of Treg cells and Treg/Th17 ratio were significantly lower than those in the control group(P<0.05).The expression rate of RORγt mRNA in EBV infection group was significantly higher than that in the control group,but the expression rate of Foxp3 mRNA was significantly lower than that in the control group(P<0.05).The IL-17 level in EBV infection group was significantly higher than that in the control group,and TGF-β1 level was significantly lower than the control group(P<0.05).Conclusion The incidence of EBV infection in children is closely related to the imbalance of Th17 cells and Treg cells as well as changes of related characteristic cytokines.

关 键 词：TH17细胞 TREG细胞 细胞因子 EB病毒 相关性 

分 类 号：R512.7[医药卫生—内科学] R446.113[医药卫生—临床医学]