血管紧张素受体1相关受体蛋白在出生后小鼠耳蜗毛细胞和螺旋神经元内的动态表达  

Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons

作　　者：尹海燕 王择雨 郝长江 张慧[1] 王凤琴[1] 李秀国[2] 陈京[3] YIN Hai-yan;WANG Ze-yu;HAO Chang-jiang;ZHANG Hui;WANG Feng-qin;LI Xiu-guo;CHEN Jing(Department of Histology and Embryology,Jining Medical University,Shandong Jining 262067,China;Otolaryngology-Head and Neck Surgery,Jining First People’s Hospital,Shandong Jining 272011,China;Institute of Neurobiology,Jining Medical University,Shandong Jining 262067,China)

机构地区：[1]济宁医学院组织学与胚胎学教研室,山东济宁262067 [2]济宁市第一人民医院耳鼻喉科,山东济宁272011 [3]济宁医学院神经生物研究所,山东济宁262067

出　　处：《解剖学报》2021年第2期175-181,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(82000979,31271243);济宁医学院贺林院士新医学临床转化工作站科研基金(JYHL2019MS13);大学生创新实验项目(S201910443020,cx2020125)。

摘　　要：目的探讨血管紧张素受体1相关受体蛋白(APJ)在出生后C57BL/6小鼠耳蜗毛细胞和螺旋神经元成熟过程的时空表达规律。方法分别取出生后7 d(P7)、P14、P28和出生后2月(P2M)时间点各11只C57BL/6小鼠的耳蜗组织,Real-time PCR、Western blotting和免疫荧光检测APJ在不同时间点耳蜗毛细胞和螺旋神经元表达。结果Real-time PCR、Western blotting结果显示,APJ在P7~P2M的耳蜗组织内表达呈上升趋势;免疫荧光结果显示,APJ在P14小鼠毛细胞和螺旋神经元中的表达明显上升,并持续至P2M;P14小鼠APJ在毛细胞和螺旋神经元内的表达呈现出从底转到顶转的下降趋势。结论APJ在出生后的小鼠耳蜗成熟过程中表现出独特的时空模式,可能参与耳蜗成熟和听力的形成和维持。Objective To study the temporal and spatial expressions of G protein-coupled receptor,putative receptor protein related to angiotensin type 1 receptor(APJ),in mammal cochlea postnatal development.Methods The cochlear tissues of each group 11 C57BL/6 mice at postnatal day 7(P7),P14,P28 and postnatal month 2(P2M)were taken out under a stereo microscope.Real-time PCR,Western blotting and immunouorescent staining were used to detect the expressions of APJ in hair cells and spiral ganglion neurons.Results The expression pattern of APJ in cochleae showed an upward trend during the period from P7 to P2M.The temporal expressions of APJ in hair cells and spiral ganglion neurons increased obviously at P14 and P2M.The spatial expression patterns of APJ in hair cells and spiral ganglion neurons followed a declined gradient from base turns to apex turns at P14.Conclusion APJ expression exhibits a specific spatial and temporal pattern during mouse cochlea postnatal development,and may play a role in cochleae maturation and hearing formation.

关 键 词：血管紧张素受体1相关受体蛋白 毛细胞 螺旋神经元 免疫荧光 小鼠 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学]