石菖蒲挥发油对炎症痛大鼠基底外侧杏仁核中胶质纤维酸性蛋白和即刻早期基因表达的影响  

Effects of volatile oil from Acori Graminei Rhizoma on glial fibrillary acidic protein and immediate early genes expressions in the basal lateral amygdala of the inflammatory pain rats

作　　者：李诗琪 杨翠珠 刘鸿庆 张春梅 田素民 刘靖 马宇昕 李国营 LI Shi-qi;YANG Cui-zhu;LIU Hong-qing;ZHANG Chun-mei;TIAN Su-min;LIU Jing;MA Yu-xin;LI Guo-ying(Department of Human Anatomy and Histology and Embryology,School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学生命科学与生物制药学院人体解剖学与组织学胚胎学系,广州510006

出　　处：《解剖学报》2021年第2期189-195,共7页Acta Anatomica Sinica

基　　金：广东省自然科学基金(2018A0303130073);广州市科技计划项目科技创新人才专项-珠江科技新星项目(201710010002)。

摘　　要：目的通过注射完全弗氏佐剂(CFA)构建炎症痛大鼠模型,探讨石菖蒲挥发油对炎症痛大鼠基底外侧杏仁核(BLA)中神经胶质纤维酸性蛋白(GFAP)和即刻早期基因(c-fos)表达的影响。方法成年SD雄性大鼠36只,随机分为6组:对照(control)组、假手术(sham)组、CFA组、CFA+5 g/(kg·d)石菖蒲挥发油组、CFA+10 g/(kg·d)石菖蒲挥发油组、CFA+20 g/(kg·d)石菖蒲挥发油组,每组各6只动物,于灌胃21 d后取材。采用免疫荧光及Western blotting技术检测各组大鼠BLA中GFAP和c-fos表达量的变化。结果免疫荧光及Western blotting结果均显示,与control组相比,CFA组大鼠BLA中GFAP及c-fos阳性表达均显著增多(P<0.01);与CFA组相比,石菖蒲挥发油处理组GFAP和c-fos阳性表达均降低,且表达量呈剂量依赖性递减(P<0.01);其中,高剂量组GFAP和c-fos阳性表达的下降趋势较低剂量组相比更为显著(P<0.01)。结论石菖蒲挥发油可减少CFA诱导的炎症性疼痛大鼠BLA中星形胶质细胞表达,并进一步抑制c-fos的表达。Objective To construct a rat model of inflammatory pain by injecting complete Freund’adjuvant(CFA)to study effects of volatile oil of Acori Graminei Rhizoma on the expression of glial fibrillary acidic protein(GFAP)and immediate early gene c-fos in the basal lateral amygdale(BLA)of the inflammatory pain rats.Methods Thirty-six adult male SD rats were randomly divided into 6 groups:control group,sham group,CFA group,CFA+5 g/(kg·day)volatile oil of Acori Graminei Rhizoma group,CFA+10 g/(kg·day)volatile oil of Acori Graminei Rhizoma group,CFA+20 g/(kg·day)volatile oil of Acori Graminei Rhizoma group,six rats in each group were taken gavage for 21 days.Immunofluorescence and Western blotting methods were used to detect the expressions of GFAP and c-fos in the BLA of all rats.Results Immunofluorescence and Western blotting results showed that compared with the control group,the positive expression of GFAP and c-fos in the BLA of the CFA rats were significantly increased(P<0.01).After treatment of the volatile oil from Acori Graminei Rhizoma,the positive expressions of GFAP and c-fos were reduced compared to the CFA group,as well as the expression levels were decreased in the dose-dependent manner(P<0.01).Compared with the low dose group,the positive expression of GFAP and c-fos of high dose group were decreased significantly(P<0.01).Conclusion The volatile oil fraction from Acori Graminei Rhizoma could reduce the expressions of GFAP and c-fos the BLA of CFA-induced chronic inflammatory pain model rats.

关 键 词：石菖蒲挥发油 基底外侧杏仁核 炎症痛 胶质纤维酸性蛋白 即刻早期基因 免疫荧光染色 免疫印迹法 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]