心脉隆注射液改善大鼠离体心脏心肌缺血/再灌注损伤  

Xinmailong injection improving myocardial ischemia reperfusion injury in isolated-heart of rats

作　　者：王永洁 姜永良 林志[1] 刘燃[1] 姜雪梅[1] 陆地 孙林[1] WANG Yong-jie;JIANG Yong-liang;LIN Zhi;LIURan;JIANG Xue-mei;LU Di;SUN Lin(Department of Cardiology Third Ward,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China;Technology Transfer Center,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学第二附属医院心血管内科三病区,昆明650101 [2]昆明医科大学技术转移中心,昆明650500

出　　处：《解剖学报》2021年第2期277-283,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(31760292,81560050);云南省科技厅-昆医大应用基础研究联合专项[2018FE001(-053),2019FE001(-069)];云南省高校冠心病基础与临床研究科技创新团队。

摘　　要：目的探讨心脉隆注射液对大鼠离体心脏心肌缺血/再灌注(I/R)损伤的保护作用。方法SPF级SD大鼠60只,随机分为6组,包括假手术(sham)组、I/R处理组以及心脉隆注射液处理组(50 mg/L、100 mg/L、200 mg/L、400 mg/L),每组10只;运用Langendorff灌流系统制作大鼠离体心脏I/R损伤模型,平衡时期持续灌注K-H液,离体心脏模型缺血复灌后灌注心脉隆注射液;多导生理转换仪Lab Chart 8.0数据采集分析软件,同步记录左心室发展压(LVDP),描记左心室内压最大上升和下降速率(±dp/dtmax)以及心率(HR)。采用生化仪检测不同组冠状动脉流出液中心肌酶学,肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、肌钙蛋白-Ⅰ(TnI)和肌红蛋白(MYO);实时测量冠状动脉流量,运用TTC染色了解心肌梗死面积,采用Western blotting检测Bax、Bcl-2在各组中的表达。结果心脉隆注射液能够减轻心肌缺血再灌注损伤大鼠LVDP、最大上升速率+dp/dt、最大下降速率-dp/dt的下降,减少心肌缺血再灌注损伤大鼠心肌细胞酶的释放,减少心肌梗死面积,增加心脏冠状动脉流量,引起心率增快以及一定的抑制心肌凋亡作用。结论心脉隆注射液能够减轻心肌I/R损伤引起的心功能损害。Objective To observe the protective effect of Xinmailong injection(XML)on myocardial ischemia reperfusion(I/R)injury in rats model.Methods The levels of SPF 60 successful acute myocardial ischemia rat models were randomly divided into control group,I/R group and XML group(Different drug concentrations 50 mg/L,100 mg/L,200 mg/L,400 mg/L).The control group was continuously given K-H nutrient solution for 2 hours,the other group was given respective different drug concentrations or K-H nutrient solution by Langendorff isolated heart perfusion method after ischemia 30 minutes.The following indicators were detected:Hemodynamics included heart rate(HR),left ventricular development pressure(LVDP)and±dp/dtmax;the myocardial enzymes included creatine kinase(CK),creatine minaseMB(CK-MB),troponin-Ⅰ(Tn-Ⅰ)and myoglobin(MYO),coronary flow and myocardial infarct size were observed.The expression of Bax and Bcl-2 in each group was detected by Western blotting and immunofluorescence.Results Xinmailong injection could alleviate LVDP and±dp/dtmax;decrease the release of CK,CK-MB,Tn-Ⅰ,MYO and the shrink area of myocardial infarction;increase the coronary flow;enhance heart rate fast and certain inhibition of myocardial apoptosis.Conclusion Xinmailong injection can alleviate the damage of cardiac function caused by myocardial ischemia/reperfusion injury.

关 键 词：心脉隆注射液 离体心脏 缺血再灌注 损伤保护 免疫印迹法 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]