醛固酮合成酶基因启动子-344T/C多态性与青海省汉族孕妇子痫前期的相关性  

Association between the polymorphism of aldosterone synthase gene promoter-344T/C and preeclampsia in Qinghai Province

作　　者：李文佳 王茹 段倩 李长兴 李红荣 李建华 LI Wen-jia;WANG Ru;DUAN Qian;LI Chang-xing;LI Hong-rong;LI Jian-hua(Department of Human Anatomy,Basic Medicine Science,Medical College of Qinghai University,Xining 810001,China;Department of Surgery,Gansu Wuwei Tumor Hospital,Gansu Wuwei 733000,China)

机构地区：[1]青海大学医学院基础医学部人体解剖学教研室,西宁810001 [2]甘肃省武威肿瘤医院外科,甘肃武威733000

出　　处：《解剖学报》2021年第2期306-310,共5页Acta Anatomica Sinica

基　　金：青海省科技厅项目(2015-ZJ-746,2019-SF-134)。

摘　　要：目的探讨青海省汉族孕妇子痫前期(PE)与醛固酮合成酶基因(CYP11B2)启动子区单核苷酸多态性的关系。方法选择青海省PE患者149例,正常妊娠对照组155例,应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法,分析PE组和对照组CYP11B2-344T/C多态性分型并测序验证。结果PE组基因型频率TT型、CT型及CC型分别为43.0%、45.6%和11.4%,对照组分别为51.0%、45.1%和3.9%。PE组和对照组醛固酮合酶基因-344T/C位点等位基因T和C频率分布具有差异性(P<0.05),PE组C等位基因频率高于对照组(χ^(2)=4.354,P<0.05,OR=0.691,95%CI=0.49~0.98)。结论CYP11B2-344T/C多态性与青海省子痫前期有关,C等位基因可能是子痫前期的易感基因。Objective To investigate the relationship between preeclampsia(PE)and polymorphism of aldosterone synthase gene(CYP11B2)promoter region-344T/C in Qinghai Province.Methods A total of 120 PE subjects and 155 normal pregnancy subjects were studied.The genotype of CYP11B2 was analyzed by polymerase chain reaction fragment length polymorphism(PCR-RFLP).The mutation was confirmed by sequencing.Results The frequencies of CYP11B2 TT,CT and CC genotype in the PE group were 43.0%,45.6%,and 11.4%,and in the control group were 51.0%,45.1%,and 3.9%,respectively.There was difference in frequency distribution of CYP11B2 genotype between the PE and control groups.The frequency of C allele in the PE group was higher than the control group(χ^(2)=4.354,P<0.05,OR=0.691,95%CI=0.49-0.98).Conclusion The CYP11B2-344T/C polymorphism is related to preeclampsia in Qinghai Province,and the C allele may be a susceptibility gene of preeclampsia.

关 键 词：青海 醛固酮合酶 子痫前期 基因多态性 限制性片段长度多态性聚合酶链反应 孕妇 

分 类 号：R3[医药卫生—基础医学]