miR-199b-3p介导mTOR信号通路促进前列腺癌增殖  

The effect of mir-199b-3p in prostate cancer via m TOR signaling pathway

作　　者：孔源 宫云昭[2] 吴涛[1] 王莹[3] KONG Yuan;GONG Yun-zhao;WU Tao;WANG Ying(Department of Imaging,the Second Affiliated Hospital,Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Department of Orthopedics,the Second Affiliated Hospital,Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;Engineering and Technology Center of TCM Innovation,Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学附属第二医院影像科,辽宁沈阳110032 [2]辽宁中医药大学附属第二医院骨科,辽宁沈阳110032 [3]辽宁中医药大学中医药创新工程技术中心,辽宁沈阳110032

出　　处：《解剖科学进展》2021年第2期134-137,共4页Progress of Anatomical Sciences

基　　金：辽宁省中央引导地方科技发展专项项目(2017105002);辽宁省自然科学基金指导计划项目(20180550767);沈阳市科技计划项目(17-139-8-00)。

摘　　要：目的探讨miR-199b-3p介导mTOR信号通路在前列腺癌中的作用。方法收集前列腺癌患者的组织样本,Real time-qPCR检测miR-199b-3p及mTOR的表达,荧光素酶报告基因分析miR-199b-3p与mTOR之间的作用,miR-199b-3p沉默及过表达质粒转染DU-145细胞,Western blot检测细胞内mTOR信号通路相关因子的蛋白表达。给予DU-145细胞脱甲基转移酶抑制剂AZA,Real time-qPCR检测细胞中miR-199b-3p及mTOR mRNA的表达。建立DU-145细胞荷瘤小鼠模型,尾静脉注射AZA,通过核磁共振检测肿瘤的大小。结果 miR-199b-3p在前列腺癌细胞中表达水平降低,mTOR水平显著升高,脱甲基转移酶抑制剂AZA使miR-199b-3p表达增多,抑制肿瘤的生长。结论 miR-199b-3p通过mTOR信号通路抑制前列腺癌的生长。Objective To investigate the mechanism of miRNA in prostate cancer via mTOR signaling pathway. Methods Real-time qPCR was used to detect the expression of miR-199 b-3 P and mTOR in prostate cancer tissue of patients. Luciferase reporter gene was used to analyze the effect of MiR-199 b-3 P and mTOR. miR-199 b-3 p silencing and overexpression plasmid was transfected into DU-145 cells respectively, and the expression of mTOR protein was detected by Western blot. The expression of miR-199 b-3 p and mTOR were measured by real-time qPCR in DU-145 cells. The tumor size was measured by magnetic resonance imaging(MRI) using DU-145 tumor-bearing mice with AZA injected intravenously into the tail. Results The expression level of miR-199 b-3 p was decreased, but increased for mTOR protein in prostate cancer cells compared to normal tissue, and AZA caused miR-199 b methylation and increased expression of miR-199 b-3 p, inhibited tumor growth. Conclusion miR-199 b-3 p inhibits the growth of prostate cancer via the mTOR signaling pathway.

关 键 词：前列腺癌 微小RNA 雷帕霉素的哺乳动物靶基因 脱甲基转移酶抑制剂 

分 类 号：R593.22[医药卫生—内科学]