雷公藤红素对前列腺癌DU-145细胞生物学行为的影响及机制研究  

Effect of triptolide on biological behavior of prostate cancer DU-145 cells and its mechanism

作　　者：孙强 王科峰[2] 费翔[2] 齐漫龙[3] SUN Qiang;WANG Ke-feng;FEI Xiang;QI Man-long(No.5 Surgery Division,Fengcheng Central Hospital,Fengcheng 118100;Department of Urology,Shengjing Hospital of China Medical University,Shenyang 110004;Department of Clinical Genetics,Shengjing Hospital of China Medical University,Shenyang 110004,China)

机构地区：[1]辽宁省凤城市中心医院外五科,辽宁凤城118100 [2]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [3]中国医科大学附属盛京医院临床遗传科,辽宁沈阳110004

出　　处：《解剖科学进展》2021年第2期183-185,190,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(2019-MS-371)。

摘　　要：目的探讨雷公藤红素对前列腺癌DU-145细胞生物学行为的影响及可能机制。方法体外培养DU-145细胞,分别以2.5、5.0、10.0μmol/L雷公藤红素处理,对照组细胞以等量0.9%NaCl处理。给药48 h后,MTT实验与平板克隆实验检测DU-145细胞增殖能力;Transwell实验检测DU-145细胞迁移和侵袭能力;流式细胞术检测DU-145细胞的凋亡;Western blot检测细胞周期蛋白Dl(cyclin D1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的蛋白表达水平。结果不同浓度雷公藤红素处理后,DU-145细胞的细胞活力降低、迁移和侵袭能力降低,细胞凋亡率明显升高,DU-145细胞cyclin D1、VEGF与MMP-9的表达水平均明显降低(P<0.01)。结论雷公藤红素抑制人前列腺癌DU-145细胞增殖、侵袭及迁移,促进凋亡。Objective To investigate the effect of triptolide on the biological behavior of prostate cancer DU-145 cells and its possible mechanism. Methods DU-145 cells were cultured in vitro and treated with 2.5 μ mol/L, 5.0 μ mol/L and 10.0 μ mol/L triptolide respectively. The cells in control group were treated with the same amount of 0.9% NaCl. After 48 hours of administration, the proliferation of DU-145 cells was detected by MTT assay and plate cloning assay. The migration and invasion of DU-145 cells were detected by Transwell assay. The apoptosis of DU-145 cells was detected by flow cytometry. The expression of cyclin D1, vascular endothelial growth factor(VEGF) and matrix metalloproteinase-9(MMP-9) were detected by Western blot. Results After treatment with different concentrations of triptolide, the cell viability, migration and invasion ability of DU-145 cells were decreased, the apoptosis rate was significantly increased, and the expression levels of cyclin D1, VEGF and MMP-9 were significantly decreased(P<0.05). Conclusion Triptolide can inhibit the proliferation, invasion and migration of human prostate cancer DU-145 cells, and promote apoptosis.

关 键 词：雷公藤红素 前列腺癌 Du-145细胞 增殖 迁移 侵袭 凋亡 

分 类 号：R593.22[医药卫生—内科学]