姜黄素通过miR-182-TLR4途径抑制缺氧诱导的小胶质细胞炎症反应  

Curcumin inhibits hypoxia induced microglial inflammation via mi R-182-TLR4 pathway

作　　者：郑敏[1] 黄蕾[1] 孙明立 董丹 ZHENG Min;HUANG Lei;SUN Ming-li;DONG Dan(Department of Neurology,Shenyang 242 Hospital,Shenyang 110034;Department of Pharmacology,School of Pharmacy,China Medical University,Shenyang 110122;Department of Pathophysiology,College of Basic Medical Science,China Medical University,Shenyang 110122,China)

机构地区：[1]沈阳二四二医院神经内科,辽宁沈阳110034 [2]中国医科大学药学院药理学教研室,辽宁沈阳110122 [3]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2021年第2期194-197,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅青年育苗项目(QN2019033)。

摘　　要：目的研究姜黄素对缺氧诱导的小胶质细胞炎症反应的抑制作用及机制。方法采用CCK8法检测姜黄素对BV2小胶质细胞的作用浓度。利用转染试剂Lipofectamine 2000进行小胶质细胞的miR-182inhibitor转染,转染后24h通过氧糖剥夺处理模拟缺血缺氧环境诱导BV-2小胶质细胞活化建立炎症模型,缺氧诱导前给予姜黄素(10μmol/L)干预2h。通过Western blot检测TLR4蛋白表达,RT-qPCR检测miR-182表达,ELISA法检测细胞培养上清液中炎症因子TNF-α、IL-6和IL-1β水平。结果姜黄素显著抑制缺氧诱导BV-2小胶质细胞分泌炎症因子TNF-α、IL-6和IL-1β水平,促进miR-182表达,抑制TLR4表达;miR-182 inhibitor可部分逆转姜黄素对缺氧诱导BV-2小胶质细胞分泌炎症因子和TLR4表达的抑制作用。结论姜黄素通过miR-182-TLR4通路抑制缺氧诱导的小胶质细胞炎症反应。Objective To study the inhibitory effect and mechanism of curcumin on hypoxia induced inflammation of microglia. Methods CCK8 assay was used to detect the concentration of curcumin on BV2 microglia. Microglia were transfected with miR-182 inhibitor by Lipofectamine 2000. 24 h after transfection, the activation of BV-2 microglia was induced by oxygen glucose deprivation(OGD) to establish the inflammatory model, and the transfected cell wastreated by curcumin for 2 hbeforeOGD. The expression of TLR4 Protein was detected by Western blot, miR-182 by RTqPCR, TNF-α, IL-6 and IL-1 β in the supernatant of cell culture were detected by ELISA. Results Curcumin significantly inhibited the secretion of TNF-α, IL-6 and IL-1 β, promoted the expression of miR-182 and inhibited the expression of TLR4. miR-182 inhibitor partially reversed the inhibition of curcumin on the secretion of inflammatory factors and TLR4 expression of BV-2 microglia induced by hypoxia. Conclusion Curcumin inhibits the inflammation of microglia induced by hypoxia via miR-182-TLR4 pathway.

关 键 词：miR-182 TOLL样受体4 姜黄素 小胶质细胞 炎症反应 

分 类 号：R593.22[医药卫生—内科学]