LncRNA OIP5-AS1通过下调miR-421表达促进骨肉瘤细胞增殖  

Lnc RNA OIP5-AS1 inhibits the proliferation of osteosarcoma cells through sponge adsorption of mi R-421 and up-regulation of Notch1 expression

作　　者：于洪德[1] 尚静波[1] 沙华 李仁波[1] 周伟[1] 齐林 张硕[3] YU Hong-de;SHANG Jing-bo;SHA Hua;LI Ren-bo;ZHOU Wei;QI Lin;ZHANG Shuo(Spinal Trauma Ward,The Third People′s Hospital of Dalian,Dalian 116021;Emergency Department,Dalian Central Hospital,Dalian 116033;Department of Orthopaedics,Shengjing Hospital,China Medical University,Shenyang 110004,China)

机构地区：[1]大连市第三人民医院脊柱创伤病房,辽宁大连116021 [2]大连市中心医院急诊科,辽宁大连116033 [3]中国医科大学附属盛京医院骨科,辽宁沈阳110004

出　　处：《解剖科学进展》2021年第2期202-205,210,共5页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(20180140211)。

摘　　要：目的探讨lncRNA OIP5-AS1通过调节miR-421对骨肉瘤癌细胞增殖能力的影响。方法通过生物信息学网站预测miR-421是否与lncRNA OIP5-AS1结合,双荧光素酶报告基因实验进行验证。构建lncRNA OIP5-AS1过表达载体,转染细胞骨肉瘤细胞,Real time-qPCR检测miR-421的表达,CCK-8检测增殖;miR-421mimics转染后,检测lncRNA OIP5-AS1和miR-421对mTOR mRNA及蛋白表达的影响。结果 lncRNA OIP5-AS1过表达促进骨肉瘤细胞体外增殖活性,下调miR-421的表达;lncRNA OIP5-AS1与miR-421结合,且lncRNA OIP5-AS1通过靶向miR-421抑制骨肉瘤细胞增殖;同时,lncRNA OIP5-AS1通过结合miR-421上调mTOR的表达。结论lncRNA OIP5-AS1过表达通过下调miR-421表达促进骨肉瘤细胞增殖。Objective To investigate the effect of lncRNA OIP5-AS1 on the proliferation of cancer cells in osteosarcoma by regulating miR-421. Methods The bioinformatics website was used to predict the binding of miR-421 to lncRNA OIP5-AS1, verified by double luciferase reporter gene experiment. LncRNA OIP5-AS1 overexpression vector was constructed and transfected into osteosarcoma cells, miR-421 expression was detected by Real time-qPCR, proliferation was detected by CCK-8, and co-transfection with miR-421 mimics was used to detect the effect of lncRNA OIP5-AS1 and miR-421 on mTOR mRNA and protein expression. Results Overexpression of lncRNA OIP5-AS1 promoted the proliferation activity of osteosarcoma cells in vitro and down-regulated the expression level of miR-421;lncRNA OIP5-AS1 combined with miR-421, and lncRNA OIP5-AS1 inhibited the proliferation of osteosarcoma cells via targeting miR-421;at the same time, lncRNA OIP5-AS1 up-regulated mTOR level by combining miR-421. Conclusion Overexpressed lncRNA OIP5-AS1 promotes the proliferation of osteosarcoma cells by down-regulating the expression of miR-421.

关 键 词：骨肉瘤 微小RNA 长链非编码RNA miR-421 细胞增殖 

分 类 号：R593.22[医药卫生—内科学]