经颅刺激结合康复训练对脑缺血再灌注小鼠神经损伤的影响及可能机制  

Effect of transcranial stimulation combined with rehabilitation training on nerve injury induced by cerebral ischemia-reperfusion in mice and its possible mechanism

作　　者：侯丹丹[1] 于璐[2] 赵珊珊[1] HOU Dan-dan;YU Lu;ZHAO Shan-shan(Internal Medicine-Neurology,The First Affiliated Hospital of China Medical University,Shenyang 110001,China;Department of rehabilitation medicine,The First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]中国医科大学附属第一医院康复医学科,辽宁沈阳110001

出　　处：《解剖科学进展》2021年第2期206-210,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年基金(81401002)。

摘　　要：目的探讨经颅刺激结合康复训练对脑缺血再灌注小鼠神经损伤的影响及可能机制。方法 80只C57BL/6J野生型小鼠随机分为对照组、假手术组、模型组、治疗组与中和组,采用血管夹夹闭小鼠颈总动脉构建小鼠脑缺血再灌注损伤模型,对各组小鼠进行神经功能评分,测定脑水含量、脑钠离子含量变化,TTC染色法测定脑梗死体积,TUNEL检测脑组织细胞凋亡,qPCR与Western blot检测各组小鼠脑组织SDF-1、p-Akt的表达变化。结果与模型组相比,治疗组小鼠的三项九分法评分明显升高,五级四分法评分、钠离子含量、脑水占比、梗死面积及TUNEL阳性率均明显降低(P<0.05);血清SDF-1的含量及脑组织SDF-1、p-Akt的表达水平均明显升高(P<0.05)。给予SDF-1中和抗体治疗后,中和组小鼠三项九分法评分明显低于治疗组,五级四分法评分、钠离子含量、脑水占比及梗死面积均明显高于治疗组(P<0.05)。结论经颅刺激结合康复训练通过促进SDF-1的表达激活PI3K/Akt信号通路,从而发挥脑缺血再灌注小鼠的保护作用。Objective To investigate the effect of transcranial stimulation combined with rehabilitation training on nerve injury in mice with cerebral ischemia-reperfusion and its possible mechanism. Methods 80 C57 BL/6 J wild-type mice were randomly divided into control group, sham operation group, model group, treatment group and neutralization group. The cerebral ischemia-reperfusion injury model was established by clamping the common carotid artery of mice. The neurological function score, brain water content and brain sodium ion content of mice in each group were measured. The cerebral infarction volume was measured by TTC staining. The apoptosis of brain tissue was detected by TUNEL. The expression of SDF-1 and p-Akt in brain tissue of mice in each group was detected by qPCR and Western blot. Results Compared with the model group, the scores of three items nine score in the treatment group were significantly increased, the score of grade 5, the content of sodium ion, the proportion of brain water, the infarct size and the positive rate of TUNEL were significantly decreased(P<0.05);the content of SDF-1 in serum and the expression levels of SDF-1 and p-Akt in brain tissue were significantly increased(P<0.05). After treatment with SDF-1 neutralizing antibody, the scores of three items nine score in the neutralization group were significantly lower than those in the treatment group, and the score of grade 5, sodium ion content, the proportion of brain water and infarct size in the neutralization group were significantly higher than those in the treatment group(P<0.05). Conclusion Transcranial stimulation combined with rehabilitation training can activate PI3 K/Akt signaling pathway by promoting the expression of SDF-1, and play a protective role in cerebral ischemiareperfusion mice.

关 键 词：脑缺血再灌注 经颅刺激 康复训练 SDF-1 PI3K/AKT 

分 类 号：R593.22[医药卫生—内科学]