人羊膜上皮细胞抑制TGF-β1诱导的LX-2细胞纤维化  

The effect of h AEC on migration, proliferation and collagen type Ⅰexpression in LX-2 cells induced by TGF-β1

作　　者：张焜 赵峰[1] 张晶 聂宏光[1] 庞希宁[1,2] ZHANG Kun;ZHAO Feng;ZHANG Jing;NIE Hong-guang;PANG Xi-ning(Department of Stem Cell and Regeneration Medicine,Basic Medical College,China Medical University,Shenyang 110122;Shenyang Amnion Bioengineering and Technology R&D Center,Shenyang 110015,China)

机构地区：[1]中国医科大学基础医学院干细胞与再生医学教研室,辽宁沈阳110122 [2]沈阳市艾米奥生物工程技术研发中心有限公司,辽宁沈阳110015

出　　处：《解剖科学进展》2021年第2期214-217,共4页Progress of Anatomical Sciences

基　　金：沈阳市干细胞与再生医学重点实验室项目(20-204-4-31)。

摘　　要：目的探索人羊膜上皮细胞条件培养基(hAEC-CM)对使用转化生长因子β1(TGF-β1)诱导的人肝星状细胞(LX-2)迁移、增殖和Ⅰ型胶原蛋白表达水平的影响及可能的机制。方法采用Transwell迁移试验检测诱导后的LX-2细胞迁移能力的变化。MTS检测诱导后的LX-2细胞增殖能力的变化。Real-time PCR与Western blot分别检测诱导后LX-2细胞Ⅰ型胶原蛋白mRNA和蛋白表达的变化。结果人羊膜上皮细胞条件培养基显著抑制转化生长因子β1诱导的LX-2细胞迁移、增殖,显著降低Ⅰ型胶原蛋白的表达水平。结论人羊膜上皮细胞抑制肝纤维化与抑制肝星状细胞迁移、增殖和Ⅰ型胶原蛋白表达相关。Objective To investigate the effect of human amniotic epithelial cell conditioned medium(hAECCM) on the migration, proliferation and type I collagen expression of human hepatic stellate cell line(LX-2) induced by transforming growth factor β1(TGF-β1). Methods Transwell migration assay was used to detect the the migration ability of induced LX-2 cells. MTS was used to detect the proliferation ability of induced LX-2 cells. Real-time PCR and Western blot were used to detect the expression of type I collagen mRNA and protein in LX-2 cells. Results hAEC-CM could significantly inhibit the migration and proliferation of induced LX-2 cells and significantly reduce the expression level of type I collagen. Conclusion The inhibition of amniotic epithelial stem cells on the migration and proliferation of hepatic stellate cells and the expression of type Ⅰ collagen may be the reason for the inhibition of hepatic fibrosis.

关 键 词：肝纤维化 肝星状细胞 条件培养基 人羊膜上皮细胞 TGF-Β1 

分 类 号：R593.22[医药卫生—内科学]