阿司匹林通过抑制NF-κB信号途经减轻缺血再灌注肾损伤  

Aspirin alleviates renal ischemia-reperfusion injury by inhibiting NF-κB signaling pathways

作　　者：丁永学 张昊[2] 孔垂泽[2] 满晓军[2] DING Yong-xue;ZHANG Hao;KONG Chui-ze;MAN Xiao-jun(Department of Urology,Liaoyang Central Hospital,Liaoyang 111000;Department of Urology,The First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]辽阳市中心医院泌尿外科,辽宁辽阳111000 [2]中国医科大学附属第一医院泌尿外科,辽宁沈阳110001

出　　处：《解剖科学进展》2021年第2期226-229,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅2019年度科学研究经费项目(QN2019008);2018年中国医科大学“青年骨干支持计划”项目(QGZ201804)。

摘　　要：目的探究阿司匹林对大鼠缺血再灌注肾损伤的保护作用及机制。方法 30只SD大鼠随机分成假手术(Sham)组、模型(IRI)组及阿司匹林(ASP)组,每组10只。血生化检测各组大鼠肾脏滤过能力;HE染色观察肾脏形态学变化;比色法检测氧化应激水平;Western Blot检测炎性因子、损伤标志物以及NF-κB信号通路相关蛋白的表达;ELISA法检测NF-κB下游的肿瘤坏死因子(TNF-α)和白介素-1β(IL-1β)的蛋白水平。结果阿司匹林可以改善IRI大鼠肾脏滤过功能,降低氧化应激水平,抑制炎症反应,减轻肾损伤,同时抑制NF-κB蛋白表达,上调IκB表达。结论阿司匹林具有保护大鼠缺血再灌注肾损伤的作用,其作用机制可能与NF-κB信号通路有关。Objective To study the protective effect and mechanism of aspirin on renal ischemia-reperfusion injury in rats. Methods Thirty SD rats were randomly divided into Sham operation(Sham) group, model(IRI) group and aspirin(ASP) group, with 10 rats in each group. The renal filtration capacity of each group of rats was detected by blood biochemistry;the morphological changes of the kidneys were observed by HE staining;the oxidative stress level was detected by kit colorimetry;Western Blot was used to detect inflammatory factors, injury markers and NF-κB signaling pathway related proteins;The protein expression level of NF-κB downstream gene;Tumor necrosis factor(TNF-α) and Interleukin-1β(IL-1β) was detected by ELISA. Results Aspirin can improve the renal filtration function, reduce the level of oxidative stress, inhibit inflammation and alleviate renal injury in IRI rats. At the same time, aspirin can inhibit the expression of NF-κB protein and up-regulate the expression of IκB. Conclusion Aspirin can protect rats from ischemia-reperfusion kidney injury, and its mechanism may be related to the NF-κB signaling pathway.

关 键 词：阿司匹林 缺血再灌注肾损伤 氧化应激 NF-ΚB信号通路 

分 类 号：R593.22[医药卫生—内科学]