针灸足三里穴对胃溃疡大鼠PLCγ-1及胃黏膜细胞活性的影响  

Acupuncture and Moxibustion at Zusanli Point Has Effect on PLCγ-1 and Gastric Mucosal Cells Activity in Rats with Gastric Ulcer

作　　者：宋淑娟 郝润春 刘君颖 SONG Shujuan;HAO Runchun;LIU Junying

机构地区：[1]武警河南省总队医院消化内科,河南郑州450000

出　　处：《新中医》2021年第7期123-126,共4页New Chinese Medicine

基　　金：河南省中医药科学研究专项课题(2018JDZX081)。

摘　　要：目的:探讨针灸足三里穴对胃溃疡大鼠磷脂酶C-γ1 (PLCγ-1)及胃黏膜细胞活性的影响。方法:将30只大鼠分为正常组、胃溃疡组及干预组,每组10只,除正常组外,其余2组建立胃溃疡模型,干预组采用针刺足三里等穴位处理。采用苏木精-伊红染色(HE)法检测各组大鼠胃组织病理;酶联免疫吸附法检测各组大鼠眼眶静脉血炎症因子;TUNEL法检测各组大鼠胃黏膜细胞凋亡率;免疫组化法检测各组大鼠胃组织PLCγ-1表达。结果:正常组大鼠胃组织无坏死,无炎症浸润;胃溃疡组胃组织排列紊乱,溃疡局部呈现扩张,并出现增生结缔组织,血管结构紊乱;与胃溃疡组比较,干预组溃疡部位炎症及坏死部位减轻,肉芽覆盖溃疡。与正常组比较,胃溃疡组大鼠血清白细胞介素-8 (IL-8)、肿瘤坏死因子-α(TNF-α)水平及胃黏膜细胞凋亡率升高(P<0.01,P<0.05),胃组织中PLCγ-1蛋白及m RNA表达降低(P<0.05)。与胃溃疡组比较,干预组大鼠血清IL-8、TNF-α水平及胃黏膜细胞凋亡率降低(P<0.01,P<0.05),胃组织中PLCγ-1蛋白及mRNA表达升高(P<0.05)。结论:针灸足三里穴能够减少胃黏膜细胞凋亡,降低炎性因子水平,且可能与激活PLCγ-1活性相关。Objective:To discuss the effect of acupuncture and moxibustion at Zusanli point(ST 36) on phospholipase C-γ1(PLC-γ1) and gastric mucosal cells activity in rats with gastric ulcer. Methods:A total of 30 rats were divided into the normal group,the gastric ulcer group and the intervention group,10 cases in each group. Except for those in the normal group,rat models with gastric ulcer were established in the other two groups,and the intervention group was treated with acupuncture at Zusanli point and so on. The pathology of gastric tissues in rats in all groups were detected by hematoxylin-eosin staining(HE),the inflammatory factors in orbital venous blood of rats by enzyme-linked immunosorbent assay,the apoptosis ratio in gastric mucosa of rats by TUNEL Assay, and the PLCγ-1 expression in gastric tissues of rats by immunohistochemistry.Results:In the normal group,there was no necrosis and inflammatory infiltration in gastric tissues of rats. In the gastric ulcer group, the gastric tissue arrangement was disordered, the ulcer area was locally dilated, and there was hyperplasia of connective tissue and vascular structural disorder. Compared with those in the gastric ulcer group, the inflammation and necrotic parts of the ulcer in the intervention group were relieved,and the ulcer was covered by granulation. Compared with those in the normal group,the levels of interleukin-8(IL-8),tumor necrosis factor-α(TNF-α) in serum and the apoptosis rate of gastric mucosal cells in the gastric ulcer group were increased(P<0.01,P<0.05),and the expressions of PLCγ-1 protein and mRNA in gastric tissues were decreased(P<0.05). Compared with those in the gastric ulcer group,the levels of IL-8 and TNF-α in serum and the apoptosis rate of gastric mucosal cells in the intervention group were decreased(P<0.01,P<0.05), and the expressions of PLCγ-1 protein and m RNA in gastric tissues were increased(P<0.05). Conclusion:Acupuncture and moxibustion at Zusanli point(ST 36) can reduce the apoptosis of gastric mucosal cells and the level

关 键 词：胃溃疡 针灸 足三里穴 胃黏膜细胞 PLCγ-1 动物实验 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]