外源IL-17抗体对金黄色葡萄球菌感染小鼠的影响  

The effect of exogenous IL-17 antibody on Staphylococcus aureus infected mice

作　　者：刘影[1] 胡志秀 计银铎 徐莉 LIU Ying;HU Zhixiu;JI Yinduo;XU Li(Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China;College of Veterinary Medicine,University of Minnesota,Twin City 55108,United States)

机构地区：[1]上海农林职业技术学院,上海201699 [2]明尼苏达大学双城校区兽医学院,明尼苏达州双子城55108

出　　处：《黑龙江畜牧兽医》2021年第7期74-76,80,155,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：上海农林职业技术学院课题项目(KY2-0000-16-08)。

摘　　要：为了证明外源白细胞介素-17(IL-17)抗体在抵御金黄色葡萄球菌皮肤感染中的重要性,试验于小鼠背部皮内接种金黄色葡萄球菌923及IL-17抗体(抗体组)或免疫球蛋白G2A(同型对照组)混合物,对接种后小鼠感染部位的伤口面积及接种后11天血液和各器官内细菌数,小鼠体重,肾脏、淋巴结和脾脏重量和器官指数进行了研究。结果表明:抗体组小鼠接种混合物后第3,5天感染部位伤口面积显著大于同型对照组(P<0.05);抗体组单位体积血液中的细菌数极显著高于同型对照组(P<0.01),单位重量心脏和肺脏中的细菌数显著高于同型对照组(P<0.05);抗体组小鼠接种混合物后第3天体增重高于同型对照组,从第5天开始到第11天同型对照组小鼠体增重均高于抗体组,但两组的体增重未达到统计学显著水平(P>0.05);两组小鼠肾脏、脾脏和淋巴结的重量和器官指数无统计学差异(P>0.05)。说明IL-17抗体与金黄色葡萄球菌混合注入小鼠背部皮内后能直接与机体产生的IL-17发生抗原抗体反应,阻碍IL-17发挥抵御金黄色葡萄球菌皮肤感染的作用,进一步证实IL-17在抗金黄色葡萄球菌皮肤感染中的重要性。In order to prove the importance of exogenous interleukin-17(IL-17) antibody in defending against Staphylococcus aureus skin infections, in this experiment the back of mice was inoculated with the mixture of Staphylococcus aureus 923 with IL-17 antibody(antibody group) or immunoglobulin G2 A(same type control group). The wound sizes of the infected site of the mouse after inoculation, the number of bacteria in the blood 11 days after inoculation, the number of bacteria in each organ, mouse body weights, the weights of the kidney, lymph node and spleen, and the organ indexes were studied. The results showed that after the mice was inoculated with the mixture, the wound size of the infected site in the antibody group was significantly larger than that of the same type control group on the 3 rd and 5 th day(P<0.05). The number of bacteria per unit volume of blood in the antibody group was extremely significantly higher than that of the same type control group(P<0.01);the number of bacteria in the heart and lungs per unit weight was significantly higher than that in the same type control group(P<0.05). The body weight gain of mice in the antibody group was higher than that in the same type control group on day 3 after inoculation with the mixture;from day 5 to day 11,the body weight gain of mice in the same type control group was higher than that of the antibody group, but the body weight gain between the two groups did not reach statistical difference(P>0.05). The weights of the kidney, spleen and lymph node and organ indexes were not statistically significant between the two groups(P>0.05). The results suggested that IL-17 antibody could directly react with IL-17 produced by the body after being mixed with Staphylococcus aureus and being injected into the back skin of mice, preventing IL-17 from playing the role of resisting Staphylococcus aureus skin infection, and further confirmed the importance of IL-17 in resisting Staphylococcus aureus skin infection.

关 键 词：金黄色葡萄球菌 IL-17抗体 皮内接种 伤口面积 细菌数 体重 

分 类 号：S852.611[农业科学—基础兽医学]