禽致病性大肠杆菌O78对边鸡血清细胞因子水平的影响  

The effect of avian pathogenic Escherichia coli O on serum cytokine level of Bian chicken

作　　者：张翀 陈玉洁[1] ZHANG Chong;CHEN Yujie(Vocational and Technical College,Inner Mongolia Agricultural University,Baotou 014109,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014109

出　　处：《黑龙江畜牧兽医》2021年第7期90-94,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：内蒙古农业大学职业技术学院科技创新项目(2017CXTD02);内蒙古自治区高等学校科学研究项目(NJZY18065)。

摘　　要：为了研究禽致病性大肠杆菌O78对边鸡血清细胞因子水平的影响,试验将112只30日龄边鸡分为2组:对照组7个处理,每个处理8只,腹腔注射无菌生理盐水;试验组7个处理,每个处理8只,腹腔注射50%致死浓度的禽致病性大肠杆菌O78菌悬液。试验期为7 d,分别于第0,0.5,1,1.5,3,5,7天这7个不同时间点,采用观察记录法和酶联免疫吸附试验法(ELISA)对感染禽致病性大肠杆菌O78边鸡的临床症状、死亡数量、血清细胞因子[免疫球蛋白、白细胞介素(IL)、γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)]水平进行研究。结果表明:试验组边鸡感染禽致病性大肠杆菌O78后第0.5~1.5天出现临床症状;第1.5~3天临床症状加重,部分鸡死亡;第3~5天边鸡死亡数量增加,但未死亡边鸡的精神状态出现好转;第7天未再出现死亡,边鸡整体状态恢复正常。试验组第1,1.5,3天免疫球蛋白水平均极显著低于对照组(P<0.01),第1,1.5天IL-2、IL-4、IL-6水平均极显著高于对照组(P<0.01),第1,1.5,3天IL-8、IL-10、IFN-γ、TNF-α水平均极显著高于对照组(P<0.01)。试验组第0.5~1.5天免疫球蛋白水平持续降低,第1.5天降至最低水平,从第3天开始逐渐升高;第0.5~1.5天IL-2、IL-4、IL-6、IL-8、IL-10、IFN-γ、TNF-α水平持续升高,第1.5天升至最高水平,从第3天开始逐渐降低。说明边鸡感染禽致病性大肠杆菌O78后第0~7天临床症状出现规律性变化,免疫球蛋白水平先降低后升高,白细胞介素、IFN-γ和TNF-α等先升高后降低,其中第1.5天时感染最严重,第3天后开始恢复。In order to study the effect of avian pathogenic Escherichia coli O78 on the serum cytokine levels of Bian chicken, 112 30-day-old Bian chickens were divided into 2 groups. 7 treatments were in the control group, 8 chickens in each treatment, by intraperitoneal injection of sterile saline;and 7 treatments were in the test group, 8 chickens in each treatment, by intraperitoneal injection of avian pathogenic E. coli O78 suspension of 50% lethal concentration. The test period was 7 days;at 0,0.5,1,1.5,3,5,7 d 7 different time points, the clinical symptoms, number of deaths, serum cytokine immunoglobulin, interleukin(IL),interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) levels of chickens infected with avian pathogenic E. coli O78 were detected by observation recording method and enzyme-linked immunosorbent assay(ELISA). The results showed that the chickens in the test group infected with avian pathogenic E. coli O78 on the first 0.5 to 1.5 days showed clinical symptoms;the clinical symptoms were severe on the 1.5 to 3 days, and some chickens died;on the 3 to 5 days, the number of chicken deaths increased but the mental state of the undead chicken had improved;there were no dead chickens on the 7 th day, and the overall condition of the Bian chickens returned to normal. The immunoglobulin levels of the test group on day 1,1.5,and 3 were extremely significantly lower than those of the control group(P<0.01);the levels of IL-2,IL-4,and IL-6 on day 1,1.5 were extremely significantly higher than the control Group(P<0.01);the levels of IL-8,IL-10,IFN-γ,and TNF-α on day 1,1.5,and 3 were significantly higher than those of the control group(P<0.01). The immunoglobulin levels of the test group continued to decrease on day 0.5-1.5,and dropped to the lowest level on day 1.5, and gradually increased from day 3;IL-2,IL-4,IL-6,IL-8,IL-10,IFN-γ,and TNF-α levels continued to increase on day 0.5-1.5, and reached the highest level on day 1.5,and gradually decreased from day 3. The results indicated that the clinical sympto

关 键 词：禽致病性大肠杆菌 边鸡 血清 免疫球蛋白 白细胞介素 

分 类 号：S831[农业科学—畜牧学] S852.6[农业科学—畜牧兽医]