陇东绒山羊KRTAP2-1基因鉴定及其对羊绒性状影响的研究  

Identification of the Caprine KRTAP2-1 Gene and Its Effect on Longdong Cashmere Traits

作　　者：孙凯 赵孟丽 郝志云 王建清 沈继源 宋宜泽 柯娜 王继卿 SUN Kai;ZHAO Mengli;HAO Zhiyun;WANG Jianqing;SHEN Jiyuan;SONG Yize;KE Na;WANG Jiqing(Faculty of Animal Science and Technology/Gansu Key Laboratory of Herbivorous Animal Biotechnology,Gansu Agricultural University,Lanzhou,Gansu 730070)

机构地区：[1]甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室,甘肃兰州730070

出　　处：《核农学报》2021年第5期1091-1098,共8页Journal of Nuclear Agricultural Sciences

基　　金：甘肃省基础研究创新群体项目(18JR3RA190);甘肃农业大学“伏羲青年英才”培育计划项目(Gaufx-02Y02);甘肃省财政厅项目(033-041041)。

摘　　要：角蛋白关联蛋白(KAPs)是羊绒纤维的主要结构成分,但尚未在山羊基因组中鉴定出KRTAP2-1基因,本研究以人类KRTAP2-1基因为模板,在山羊基因组中进行同源性搜索,在19号染色体上发现了一个399 bp的开放阅读框。采用聚合酶链反应-单链构象多态性(PCR-SSCP)方法,在249只陇东山羊上检测到7条不同的序列(A-G)。进化树结果表明,这7条序列与人类和绵羊的KAP2-1序列有较高的同源性,表明该序列是山羊KRTAP2-1的7个等位基因。测序结果表明,KRTAP2-1基因中存在12个单核苷酸多态性(SNPs)(5个SNPs位于编码区,7个SNPs位于非编码区),以及c.-61-63delCTC和c.9395delCCG两个碱基缺失,其中c.9395delCCG引起了第32位精氨酸的移码缺失。山羊KAP2-1蛋白含有丰富的半胱氨酸、脯氨酸、苏氨酸和丝氨酸。相关性分析表明,等位基因C的存在与较小的羊绒纤维直径相关(存在:13.4±0.05μm;缺失:13.6±0.03μm;P=0.010)。本研究表明,山羊KRTAP2-1基因有丰富的多态性,可作为陇东绒山羊羊绒纤维直径选育的分子标记用于生产实践。Keratin-associated proteins(KAPs)are main structural components of cashmere fibres,while KRTAP2-1 has not been identified in goat genome.In the study,a 399 bp open reading frame on chromosome 19 of goat was found to be homologous to human KRTAP2-1 sequences.Seven unique sequences(A-G)were detected in 249 Longdong cashmere goats using polymerase chain reaction-single stranded conformation polymorphism(PCR-SSCP)analysis.These sequences have the highest homology with KAP2-1 sequences of human and sheep,suggesting that these are variants of caprine KRTAP2-1.Twelve single nucleotide polymorphisms(SNPs)and two insertion/deletion(c.-61-63 delCTC and c.9395 delCCG)were found within the gene,including five SNPs in the coding region and 7 SNPs in the non-coding region.c.9395 delCCG would result in a deletion of Arg at position 32.Caprine KAP2-1 contains high levels of cysteine,proline,threonine and serine.The presence of variant C was associated with the decrease in diameter of cashmere fibre(presence:13.4±0.05μm,absence:13.6±0.03μm,P=0.010).These results indicate that caprine KRTAP2-1 are polymorphic,and variations in caprine KRTAP2-1 are valuable to be a genetic marker for reducing cashmere fibre diameter in Longdong cashmere goats.

关 键 词：KRTAP2-1基因 山羊 聚合酶链反应-单链构象多态性(PCR-SSCP) 羊绒纤维直径 

分 类 号：S827[农业科学—畜牧学]