DSF-家族群体感应信号生物合成途径与调控机制研究进展  

Research progress in the biosynthetic pathway and regulatory mechanisms of diffusible signaling factor-family quorum sensing signals

作　　者：宋凯 周莲[1] 何亚文[1] SONG Kai;ZHOU Lian;Ya-Wen HE(State Key Laboratory of Microbial Metabolism,Joint International Research Laboratory of Metabolic and Developmental Sciences,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室教育部代谢与发育科学国际合作联合实验室,上海200240

出　　处：《微生物学通报》2021年第4期1239-1248,共10页Microbiology China

基　　金：国家自然科学基金(31972231)。

摘　　要：群体感应是微生物间相互交流的一种重要机制。Diffusible Signaling Factor (DSF)-家族群体感应信号分子存在于多种革兰氏阴性菌中,调控细菌的致病性和适应性。本文首先介绍DSF-家族群体感应信号的结构多样性与保守性、生物合成途径和两类调控机制。DSF家族群体感应信号属于一类长链不饱和脂肪酸,碳水化合物和支链氨基酸是主要合成前体;合成途径主要包括脂肪酸合成循环和兼具脱水酶和硫酯酶活性的RpfF;在黄单胞菌和伯克氏菌中分别存在2种蛋白-蛋白互作机制调控DSF生物合成。随后,综述最新相关研究结果,提出顺式-2-十二碳烯酸(BDSF)可能是野油菜黄单胞菌侵染大白菜过程中所依赖的"活体"群体感应信号。最后,讨论和展望本领域下一步值得研究的关键科学问题。Quorum sensing(QS) is one of the most important cell-cell communication mechanisms used by bacteria. DSF-family QS signals are present in a range of Gram-negative bacterial species, regulating bacterial virulence and adaptation. In this review, the structural diversity and conservation of DSF-family QS signals, their biosynthetic pathways and two regulatory mechanisms controlling DSF biosynthesis were firstly introduced. DSF-family signals are all long-chain unsaturated fatty acids, their biosynthetic precursors include carbohydrates and branched-chain amino acids;the major biosynthetic pathway include fatty acid elongation cycle and the key enzyme RpfF with both dehydratase and thioesterase activities;two regulatory mechanisms involving protein-protein interactions were identified to control DSF biosynthesis in Xanthomonas and Burkholderia, respectively. Then, the key in planta QS signal used by Xanthomonas campestris pv. campestris during its infection of Chinese cabbage was introduced. Finally, some key scientific questions in this research area were discussed and prospected.

关 键 词：黄单胞菌 群体感应 DSF-家族信号 RpfF RpfC 

分 类 号：S432.42[农业科学—植物病理学]