盆炎净片对苯酚胶浆致大鼠盆腔炎性疾病后遗症的影响  

Effect of Pen Yan Jing Tablets on SPID Induced by Phenolic Mucilage in Rats

作　　者：唐萍 张传平 陆紫琪 林娟 林宝琴[1] TANG Ping;ZHANG Chuanping;LU Ziqi;LIN Juan;LIN Baoqin(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Guangzhou Baiyun Mountain and Hutchison Whampoa Ltd.,Guangzhou 510515 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州白云山和记黄埔有限公司,广东广州510515

出　　处：《中药新药与临床药理》2021年第3期332-337,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的研究盆炎净片对苯酚胶浆诱导的盆腔炎性疾病后遗症(SPID)大鼠的治疗作用及相关机制。方法采用苯酚胶浆诱导SPID大鼠模型。将30只雌性SD大鼠随机分为假手术组、模型组、妇科千金片组(656 mg·kg^(-1))、醋酸地塞米松片组(0.31 mg·kg^(-1))和盆炎净片低、高剂量组(388、775 mg·kg^(-1)),每日1次,连续灌胃给药21 d后取材。观察大鼠子宫解剖形态学的改变,并根据Philips评分标准进行盆腔粘连程度评分;采用苏木精-伊红(HE)染色观察子宫组织病理学形态;采用蛋白质免疫印迹法(Western Blot)检测子宫组织磷酸化-核转录因子κB-p65(p-NF-κB-p65)的蛋白表达;采用免疫组化法(IHC)检测子宫组织细胞间黏附分子1(ICAM-1)的蛋白表达。结果与模型组比较,盆炎净片低、高剂量组的大鼠子宫形态得到改善,盆腔粘连评分明显降低(P<0.05),子宫组织的慢性炎症病理形态减轻,子宫组织的p-NF-κB-p65、ICAM-1蛋白表达明显下调(P<0.05,P<0.01)。结论盆炎净片可能通过下调子宫组织的p-NF-κB-p65、ICAM-1蛋白表达来发挥对SPID大鼠的治疗作用。Objective To study the effect and mechanism of Pen Yan Jing tablets on phenolic mucilage-induced sequelae of pelvic inflammatory disease(SPID)in rats.MethodsThe rat model of SPID was induced by phenolic mucilage. Thirty SD rats were randomly divided into 6 groups:Sham group,model group,low and high doses of Pen Yan Jing tablets groups(388,775 mg·kg^(-1)),Fuke Qianjin tablets group(656 mg·kg^(-1))and dexamethasone group(0.31 mg·kg^(-1)). After 21 days of oral administration,the rats were sacrificed and the tissues were harvested.The anatomic morphology of uteri was observed. The extent of pelvic adhesion was evaluated by the Philips scoring criteria. The uteri were stained with hematoxylin and eosin to observe the histological changes. The protein expressions of p-NF-κB-p65 and ICAM-1 in the uteri were detected by Western Blot and immunohistochemical method, respectively.ResultsCompared with the model group, Pen Yan Jing tablets reduced the anatomic morphological changes of uteri, the pelvic adhesion score(P<0.05) and the histological changes of chronic inflammation in uteri. The protein expressions of p-NF-κB-p65 and ICAM-1(P<0.05,P<0.01)of uteri weredown-regulated by Pen Yan Jing tablets.Conclusion Pen Yan Jingtablets alleviated the phenolic mucilage induced-SPID. Down-regulating the protein expression of p-NF-κB-p65 and ICAM-1 of uteri may be the key mechanism.

关 键 词：盆炎净片 盆腔炎性疾病后遗症 苯酚胶浆 盆腔粘连 磷酸化-核转录因子κB-p65 细胞间黏附分子1 大鼠 

分 类 号：R285.5[医药卫生—中药学]