温肾强骨丸治疗脾肾阳虚型骨质疏松症模型大鼠的药效学研究  

Study on the Pharmacodynamics of Wenshen Qianggu Pills in the Treatment of Osteoporosis Rats with Yang Deficiency of Spleen and Kidney

作　　者：王雨榕 邓强[2] 李中锋[2] 彭冉东 李军杰[1] 徐浩军 WANG Yurong;DENG Qiang;LI Zhongfeng;PENG Randong;LI Junjie;XU Haojun(Gansu University of Traditional Chinese Medicine,Lanzhou 730030 Gansu,China;Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050 Gansu,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730030 [2]甘肃省中医院,甘肃兰州730050

出　　处：《中药新药与临床药理》2021年第3期357-364,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：兰州市人才创新创业项目(2017-RC-44);白求恩·脊柱病理性骨折椎体强化治疗专项基金(BK-JP2018004)。

摘　　要：目的观察温肾强骨丸对脾肾阳虚型骨质疏松症模型大鼠的影响。方法采用去势(切除大鼠卵巢)+注射氢化可的松+番泻叶灌胃+饮食失节+劳倦过度方法联合制备脾肾阳虚型骨质疏松症大鼠模型,模型复制成功后给予温肾强骨丸连续灌胃8周。给药期间,观察并记录大鼠一般情况及体质量、肛温的变化;检测大鼠脾、肾、子宫等脏器指数;检测大鼠骨密度、骨生物力学;检测血清中抗酒石酸碱性磷酸酶(TRAP)、大鼠尿Ⅰ型胶原交联N末端肽(U-NTX)、骨碱性磷酸酶(BALP)、雌二醇(E2)、尿型胶原交联C末端肽(U-CTX)、骨钙蛋白(BGP)等骨转化指标;观察并分析椎体HE染色结果。结果温肾强骨丸能明显改善模型大鼠一般状态,增加饮食量,升高体质量、肛温,明显缓解畏寒、便溏等脾肾阳虚症状;增加模型组大鼠股骨、脊柱骨密度(P<0.05),增强股骨骨生物力学(P<0.05);增高血清BALP、E2水平(P<0.05),降低血清BGP、TRAP、U-NTX、U-CTX水平(P<0.05);增加皮质骨面积百分比、骨小梁平均厚度、骨小梁面积、骨小梁体积(P<0.05)。结论温肾强骨丸可明显改善大鼠脾肾阳虚症状,增加大鼠骨密度、骨生物力学,升高血清BALP、E2水平,降低血清BGP、TRAP、U-NTX、U-CTX水平,增加皮质骨面积百分比、骨小梁平均厚度、骨小梁面积、骨小梁体积,从而治疗脾肾阳虚型骨质疏松症。Objective To observe the effect of Wenshen Qianggu pills on osteoporosis model rats with spleen and kidney Yang deficiency.MethodsRat models of osteoporosis with spleen and kidney Yang deficiency were prepared by castrating(removing ovaries of rats)+ injecting hydrocortisone + lavaging with senna leaves + incontinence of diet+ overwork. After the models were established,the Wenshen Qianggu group of rats were treated with the drug by gavage for consecutive 8 days. During administration,the general condition,body weight and anal temperature of the rats were observed and recorded. Indexes of spleen, kidney, uterus and other organs were measured. Bone density and bone biomechanics were also measured. In addition, resistance of tartaric acid in serum alkaline phosphatase(TRAP), the rat urine Ⅰ type collagen cross-linking N terminal peptide(U-NTX), bone alkalinephosphatase(BALP),estradiol(E2),urine type collagen cross-linking C terminal peptide(U-CTX),osteocalcin(BGP),and other bone transformation indicator were detected. The results of HE staining of vertebral body wereobserved and analyzed.Results Wenshen Qianggupills can obviously improve the general state of the model rats,increase the amount of food, increase body mass and anus temperature, and obviously relieve the symptoms of spleen-kidney Yang deficiency,such as fear of cold and loose stool. Bone density of femur and spine in the modelgroup was increased(P<0.05),and biomechanics of femur was enhanced(P<0.05). Serum BALP and E2 levelswere increased(P<0.05),and serum BGP,TRAP,U-NTX and U-CTX levels were decreased(P<0.05). Thepercentage of cortical bone area,average thickness of trabecular bone,trabecular bone area and trabecular bonevolume were increased(P<0.05).Conclusion Wenshen Qianggupills can obviously improve the symptoms ofspleen and kidney Yang deficiency rats,increase the rat bone mineral density,bone biomechanics,elevated serumBALP, E2 levels, reduce serum BGP, TRAP, U-NTX, U-CTX level, increase the percentage of corticalbone area,average thickness of

关 键 词：温肾强骨丸 脾肾阳虚 骨质疏松症 骨密度 骨生物力学 药效学 大鼠 

分 类 号：R285.5[医药卫生—中药学]