二精丸对去卵巢+D-半乳糖联合Aβ_(1-40)致肾阴虚AD大鼠海马多巴胺神经功能的影响  

Effect of Erjing Pill on Hippocampal Dopaminergic Function in AD Rats with Kidney Yin Deficiency Induced by Ovariectomy + D-galactose Combined with Aβ_(1-40)

作　　者：黄丽萍[1] 邱小鹏 杨喜洋 严斐霞 燕波 官扬[1] 谢永艳 孙梦盛 周茂福[1] 陈耀辉[2] HUANG Liping;QIU Xiaopeng;YANG Xiyang;YAN Feixia;YAN Bo;GUAN Yang;XIE Yongyan;SUN Mengsheng;ZHOU Maofu;CHEN Yaohui(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;Jiangxi Provincial People’s HospitalNanchang 330006 Jiangxi,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]江西省人民医院,江西南昌330006

出　　处：《中药新药与临床药理》2021年第4期479-483,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江西省主要学科学术和技术带头人资助项目(20182BCB22005);江西省自然科学基金项目(20171BAB205083);江西省卫计委中医药科研计划项目(2016A023)。

摘　　要：目的探讨二精丸对去卵巢+D-半乳糖联合β-淀粉样蛋白_(1-40)(Aβ_(_(1-40)))致肾阴虚阿尔茨海默病(AD)大鼠海马多巴胺神经功能的影响。方法将56只雌性SD大鼠随机分为假手术组、模型组、二精丸高剂量组(9.0 g·kg^(-1))、二精丸中剂量组(4.5 g·kg^(-1))、二精丸低剂量组(2.25 g·kg^(-1))。造模大鼠在卵巢摘除手术1周后,腹腔注射D-半乳糖(100 mg·kg^(-1)),每日1次,持续7周;卵巢摘除手术4周后,双侧海马注射Aβ_(1-40)建立肾阴虚AD大鼠模型。卵巢摘除手术5周后,各组按照相应剂量开始灌胃给药,模型组与假手术组灌胃等量生理盐水,给药容量为10 mL·kg^(-1),每日1次,连续30d。末次给药1h后断头取海马组织,采用ELISA法检测大鼠海马组织多巴胺(DA)水平,采用免疫组化法检测大鼠海马CA1区多巴胺D1受体、酪氨酸羟化酶(TH)的表达情况。结果与假手术组比较,模型组大鼠的海马组织DA水平显著降低(P<0.01),海马CA1区多巴胺D1受体及TH表达明显下调(P<0.01)。与模型组比较,二精丸高剂量组大鼠的海马组织DA水平明显升高(P<0.01);二精丸各剂量组大鼠海马CA1区的D1受体表达明显上调(P<0.05,P<0.01);二精丸高、中剂量组大鼠海马CA1区的TH表达明显上调(P<0.05,P<0.01)。结论二精丸能够提高海马组织的DA水平,上调海马组织的多巴胺D1受体及TH表达,促进去卵巢+D-半乳糖联合Aβ_(1-40)致肾阴虚AD大鼠的海马多巴胺神经功能。Objective To investigate the effect of Erjing pill on hippocampal dopaminergic nerve function of Alzheimer’s disease(AD) rats with kidney yin deficiency induced by ovariectomy+D-galactose combined with amyloid β_(1-40)(Aβ_(1-40)).Methods Fifty-six female SD rats were grouped according to a random number table,namely Sham operation group,model group,Erjing pill high dose group(9.0 g·kg^(-1)),Erjing pill medium dose group(4.5 g·kg^(-1))and Erjing pill low dose group(2.25 g·kg^(-1)).One week after ovariectomy,the rats were intraperitoneally injected with D-galactose daily for 7 weeks;and then bilateral hippocampus was injected with Aβ_(1-40) 4 weeks after ovariectomy to establish kidney yin deficiency AD rat model.Rats in the Sham operation group and model group were given normal saline by gavage,and the high,medium and low dose groups of Erjing pills were given suspensions of Erjing pill by gavage,10 mL·kg^(-1),once a day for 30 days from the 6 th week after ovariectomy.One hour after the last administration,the hippocampus was decapitated and the dopamine(DA) content in the hippocampus was determined by ELISA.The expression levels of dopamine D1 receptor and tyrosine hydroxylase(TH) in hippocampus were determined by immunohistochemistry.Results Compared with the Sham operation group,the DA level,the number of D1 receptor and TH positive cells in the model group were significantly reduced(P <0.01).Compared with the model group,the high dose group of Erjing pill can significantly increase the DA level in the hippocampus of AD rats(P <0.01);the high,medium and low dose groups of Erjing pill can increase the numbers of D1 positive cells(P <0.05,P <0.01);and the high and medium dose groups of Erjing pill can significantly increase the numbers of TH positive cells in CA area of the hippocampus of AD rats(P <0.05,P <0.01).Conclusion Erjing pill may improve the learning and memory abilities of AD rats with kidney yin deficiency resulted from ovariectomy+D-galactose combined with Aβ_(1-40) by increasing the conte

关 键 词：二精丸 阿尔茨海默病 肾阴虚 去卵巢 D-半乳糖 Aβ_(1-40) 多巴胺神经功能 多巴胺D1受体 酪氨酸羟化酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]