便可通片高效液相指纹图谱研究及多指标成分定量分析  

Study on HPLC Fingerprint and Multi-indicator Quantitative Analysis of Bianketong Tablets

作　　者：万丹[1] 梁雪娟[1] 唐纯玉 唐代凤 王雄龙 肖娟[1] WAN Dan;LIANG Xuejuan;TANG Chunyu;TANG Daifeng;WANG Xionglong;XIAO Juan(Hunan Provincial Institute of Traditional Chinese Medicine,Changsha 410013 Hunan,China;Hunan Times Sunshine Pharmaceutical Corp.,Yongzhou 425000 Hunan,China)

机构地区：[1]湖南省中医药研究院,湖南长沙410013 [2]湖南时代阳光药业股份有限公司,湖南永州425000

出　　处：《中药新药与临床药理》2021年第4期540-545,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技重大专项(民口)课题-重大新药创制(2018ZX09731-015);湖南省自然科学基金项目(2020JJ9046)。

摘　　要：目的建立便可通片高效液相(HPLC)指纹图谱,同时对其中的松果菊苷、柚皮苷、新橙皮苷、芦荟苷进行含量测定。方法采用InertSustain C_(18)(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.1%冰醋酸水溶液为流动相进行梯度洗脱,流速为1 mL·min^(-1);柱温:25℃;检测波长:300 nm;进样量:10μL。结果HPLC指纹图谱共确定16个共有峰,并指认出5个共有成分,指纹图谱相似度均在0.950以上;10批样品中松果菊苷、柚皮苷、新橙皮苷、芦荟苷的平均含量分别为:0.40%、1.61%、1.75%、1.63%。结论该研究建立的方法简单、重复性好,为提高便可通片的质量控制水平提供了依据。Objective To establish the HPLC fingerprint of Bianketong tablets(BKT),and to simultaneously determine the contents of four index components(Echinacoside,Naringin,neohes peri din,Aloin).Methods The HPLC analysis was performed on a column of InertSustain C_(18)(250 mm×4.6 mm,5μm)with a column temperature of 25℃.The mobile phase was consisted of methanol-0.1%glacial acetic acid with gradient elution at the flow rate of 1.0 mL·min^(-1) and the detection wavelength was set at 300 nm,and the sample injection volume was 10μL.Results The HPLC fingerprint for 10 batches of BKT were obtained,and 16 common peaks had been identified with the similarities of more than 0.95,and 5 common components were identified.The average contents of Echinacoside,Naringin,Nehesperetin and Aloin were 0.40%,1.61%,1.75%and 1.63%,respectively.Conclusion The method established in this study is simple and reproducible,and provides a basis for improving the quality control of the BKT.

关 键 词：便可通片 高效液相法 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]