血浆外泌体miR-18a-3p调控CITED2表达对多囊卵巢综合征卵丘细胞周期的影响  

Plasma-exosomal miR-18a-3p regulates CITED2 expression to affect cumulus cells apoptosis and cell cycle distribution in polycystic ovary syndrome

作　　者：孙雪艳[1] 杨丽萍 公续红 孙振莉 Sun Xueyan;Yang Liping;Gong Xuhong;Sun Zhenli(Centre for Reproductive Medicine,Yantai Yantaishan Hospital,Yantai 264000,China)

机构地区：[1]烟台市烟台山医院生殖医学中心,264000

出　　处：《中华内分泌外科杂志》2021年第2期182-188,共7页Chinese Journal of Endocrine Surgery

基　　金：烟台市重点研发计划资助项目(2017YD018)。

摘　　要：目的探讨血浆外泌体源性miR-18a-3p靶向调节Cbp/p300结合转化激活因子含Glu/Asp丰富羧基末端域2(Cbp/P300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2,CITED2)对多囊卵巢综合征(polycystic ovary syndrome,PCOS)卵丘细胞(cumulus cells,CCs)凋亡和细胞周期的影响。方法qRT-PCR分别对PCOS患者血浆、血浆外泌体及CCs中miR-18a-3p的表达进行检测。将外泌体转染miR-18a-3p模拟物后与CCs共孵育后,采用流式细胞术检测CCs凋亡和周期分布情况。行基因本体论(gene ontology,GO)分析筛选CITED2纳入后续实验。构建pcDNA3.1-CITED载体转染至CCs后再与各组外泌体共培养,探究miR-18a-3p与CITED2联合作用对CCs凋亡、周期分布的影响。结果PCOS患者分离的血浆外泌体和CCs鉴定成功。相对非PCOS患者的血浆、血浆外泌体和CCs,PCOS患者血浆样本、外泌体、CCs中miR-18a-3p表达明显下降(均P<0.05)。CITED2在CCs中可作为miR-18a-3p的靶点受到其调控。相对于NC组,过表达miR-18a-3p抑制PCOS患者中CCs细胞的G0/G1期分布,并抑制其凋亡(均P<0.05),但该作用被CITED2部分挽救(均P<0.05)。结论血浆外泌体中携带的miR-18a-3p能促进CCs细胞周期中S期增多,从而抑制其凋亡,抑制PCOS的进展。血浆外泌体源性miR-18a-3p有望在PCOS的靶向治疗中发挥作用。Objective To investigate the effect of plasma exosome miR-18a-3p on of apoptosis and cell cycle cumulus cells(CCs)in polycystic ovary syndrome(PCOS)via targeting CITED2.Methods qRT-PCR assay was used to detect the expression of miR-18a-3p in plasma,plasma-derived exosome and CCs of patients no matter with PCOS and non PCOS.After being transfected with miR-18a-3p mimic,the exosome was co-cultured with CCs,the cell cycle distribution and apoptosis of CCs were detected by flow cytometry assays.Gene ontology(GO)analysis was performed and CITED2 was included in follow up experiments.pcDNA3.1-CITED was transfected into CCs and then co-cultured with exosome to explore the co-effect of miR-18a-3p and CITED2 on the cell cycle and apoptosis of CCs.Results The plasma-derived exosome isolated from CCs with PCOS were identified successfully,and the expression of miR-18a-3p was significantly decreased in plasma,exosome and CCs in PCOS,compared with that in non-PCOS(all P<0.05).CITED2 could be regulated as a target of miR-18a-3p in CCs.Compared with NC group,overexpression of miR-18a-3p could significantly decrease proportion of CCs cells in G0/G1 phase and inhibit their apoptosis in PCOS patients(all P<0.05).The effect of over-expressing miR-18a-3p could be partially reversed by up-regulating CITED2(all P<0.05).Conclusions Plasma exosomal miR-18a-3p has the effect to induce the S phase of CCs cells,subsequently inhibit apoptosis,then restrain the progression of PCOS.Plasma exosomal miR-18a-3p is expected to play a paramount role in the target therapy of PCOS.

关 键 词：miR-18a-3p 多囊卵巢综合症 卵丘细胞 血浆外泌体 

分 类 号：R588.6[医药卫生—内分泌]