血清中碘的测定:电感耦合等离子体-质谱法的验证  

Determination of iodine in serum:verification of inductively coupled plasma mass spectrometry

作　　者：任冰璇 万思源[1] 曲梦影 吴怀永 刘丽香[1] 申红梅[1] Ren Bingxuan;Wan Siyuan;Qu Mengying;Wu Huaiyong;Liu Lixiang;Shen Hongmei(Institute for Iodine Deficiency Disorders Control,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Harbin Medical University,Harbin 150081,China;Department of Preventive Medicine,Qiqihar Medical Universiy,Qiqihar 161006,China)

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心碘缺乏病防治研究所,150081 [2]齐齐哈尔医学院预防医学教研室,黑龙江齐齐哈尔161006

出　　处：《中华地方病学杂志》2021年第4期326-332,共7页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81872561)。

摘　　要：目的:验证电感耦合等离子体-质谱法(ICP-MS)对血清中碘含量的测定,并评价ICP-MS法与砷铈催化分光光度法测定血清碘的一致性。方法:采用ICP-MS法测定血清中的碘含量,以铼(187Re)作为内标,优化各项参数;采用ICP-MS法和砷铈催化分光光度法同时测定88份血清样本,方法学评价指标包括标准曲线的测定范围、最低检出限、精密度、准确度。并且通过组间相关分析、组内相关系数(ICC)分析、Passing-Bablok回归和Bland-Altman偏差图分析评价两种方法的一致性。结果:ICP-MS方法标准曲线的线性范围为0~300μg/L,碘浓度值与碘响应值之间呈现良好的线性相关关系,相关系数范围为0.9998~0.9999;最低检出限为1.96μg/L;血清样测定的批内和批间精密度实验的相对标准偏差(RSD)范围分别为0.2%~1.4%、0.4%~1.8%;加标回收率范围为90.44%~108.71%。88份血清样相关分析的结果显示,两种方法测定结果显著相关(r=0.934,P<0.05);ICC为0.932;Passing-Bablok回归结果显示,两种方法的偏倚差异无统计学意义(P>0.05);Bland-Altman图提示两种方法的测定结果具有一致性。结论:ICP-MS测定方法最低检出限低,精密度和准确度高,简便快速且易于操作,适用于大批量血清样本中碘含量的检测;ICP-MS法与砷铈催化分光光度法测定血清碘结果具有一致性。Objective To verify the determination method of iodine in serum by inductively coupled plasma mass spectrometry(ICP-MS)and to evaluate the consistency between ICP-MS and As3+-Ce4+catalytic spectrophotometry in determination of serum iodine.Methods Serum iodine concentration was determined by ICP-MS,187Re was used as an internal standard,and ralated parameters were optimized.Eighty-eight serum samples were simultaneously determined by ICP-MS and As3+-Ce4+catalytic spectrophotometry,and the evaluation indexes included determination range of standard curve,detection limit,precision,accuracy.In addition,we also evaluated the consistency of the two methods through inter-group correlation analysis,intra-group correlation coefficient analysis,Passing-Bablok regression and Bland-Altman analysis.Results The linear range of ICP-MS standard curve was 0-300μg/L.There was a good linear correlation between iodine concentration value and iodine response value,and the correlation coefficient range was 0.9998 to 0.9999.The detection limit of the ICP-MS method was 1.96μg/L.The relative standard deviation(RSD)ranged from 0.2%to 1.4%and from 0.4%to 1.8%for intra and inter-batch precision tests of serum samples.The recovery rate ranged from 90.44%to 108.71%.The correlation analysis of 88 serum samples showed that there was a good correlation between the two methods(r=0.934,P<0.05),and the intra-class correlation coefficient was 0.932.The results of Passing-Bablok regression showed that there was no significant difference between the two methods(P>0.05).Bland-Altman diagram suggested that the results of the two methods were consistent.Conclusions ICP-MS method has low detection limit,high precision and accuracy.ICP-MS method is simple,rapid,easy and suitable for determination of iodine in large quantities of serum samples.The results of the two methods for determining serum iodine are consistent.

关 键 词：碘 电感耦合等离子体-质谱法 方法验证 

分 类 号：R446.1[医药卫生—诊断学] O657.63[医药卫生—临床医学]