HPLC法测定苹果中游离糖组成及含量  

Determination and analysis free sugar of apple gynostemmapentaphyllum by HPLC

作　　者：许海菊[1] 李玉红[1] XU Hai-ju;LI Yu-hong(College of Chemistry and Chemical Engineering,Xianyang Normol University,Xianyang 712000,China)

机构地区：[1]咸阳师范学院化学化工学院,陕西咸阳712000

出　　处：《化学工程师》2021年第4期29-32,共4页Chemical Engineer

基　　金：陕西省自然科学基金项目(2013JM2016)。

摘　　要：采用高效液相色谱法(HPLC)测定苹果中可溶性游离糖的含量。色谱分析条件分别为:氨基色谱柱,流动相为V水∶V乙腈=20∶80,流速1mL·min^(-1),柱温50℃,进样量20μL,N_(2)压强3.5bar。葡萄糖、果糖、蔗糖的含量与峰面积有很好的线性关系。在上述条件下,测得各种苹果中果糖的含量最高,其次是葡萄糖,最低的是蔗糖,经过计算可得出洛川苹果的含糖量最高,黄苹果的含糖量最低。The composition of free sugar in apple was measured by HPLC.The chromatogram-phic conditions of HPLC were as follows,amino chromatography column,the volume ratio between water and acetonitrile is 20∶80,the flow rate was 1mL·min^(-1),the column temperature was 50°C,the sample sizes was 20μL,the nitrogen pressure was 3.5bar.There is a good linear relationship between the content of glucose,fructose and sucrose and peak area.According to the results obtained that the highest containing sugar is fructose and the lowest is sucrose in various apples.Apple of Luochuan containing sugar is highest and the yellow apple containing sugar is least in this experiment.

关 键 词：苹果 游离糖 高效液相色谱法 

分 类 号：O631[理学—高分子化学]