电化学法检测细胞中的胸腺嘧啶和胞嘧啶  

Detection of Thymine and Cytosine in Cellsby Electrochemical Method

作　　者：张树萌 李锦莲[1] 郭晓玲[1] 孟令仁 周实[1] ZHANG Shumeng;LI Jinlian;GUO Xiaoling;MENG Lingren;ZHOU Shi(College of Pharmacy,Jiamusi University,Jiamusi 154007,Heilongjiang Province,China)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《吉林大学学报（理学版）》2021年第3期690-695,共6页Journal of Jilin University:Science Edition

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划项目(批准号:UNPYSCT-2020055);黑龙江省省属高等学校基本科研业务费项目(批准号:2017-KYYWF-0591);佳木斯大学骨干人才支持项目(批准号:JMSURCGG2016_002);佳木斯大学校长创新创业基金(批准号:XZYF2018-52)。

摘　　要：用基于氧化石墨烯量子点/多壁碳纳米管/丝网印刷电极(GOQDs/MWCNTs/SPCE^(*))的电化学检测方法同时检测小鼠胚胎成纤维(BALB/c 3T3)细胞中的胸腺嘧啶和胞嘧啶,并考察富集电位、富集时间、 pH值对胸腺嘧啶和胞嘧啶标准品电化学行为的影响.实验结果表明:最佳检测条件为富集电位0,富集时间150 s,pH=7.4;电极对胸腺嘧啶和胞嘧啶的最低检测限分别为0.69,0.95μmol/L;该方法可同时灵敏检测BALB/c 3T3细胞中的胸腺嘧啶和胞嘧啶.The electrochemical detection method based on graphene oxide quantum dots/multi walled carbon nanotubes/screen printed electrode(GOQDs/MWCNTs/SPCE*)was used to simultaneously detect thymine and cytosine in BALB/c 3T3 cells.The effects of enrichment potential,enrichment time and pH values on the electrochemical behavior of thymine and cytosine were investigated.The experimental results show that the optimal detection conditions are as follows:enrichment potential is 0,enrichment time is 150 s,pH=7.4.The detection limits of thymine and cytosine are 0.69,0.95μmol/L,respectively.The electrochemical detection method can be used to detect thymine and cytosine in BALB/c 3T3 cells simultaneously.

关 键 词：电化学 细胞 胸腺嘧啶 胞嘧啶 同时检测 

分 类 号：O657.1[理学—分析化学]