反复种植失败患者种植期子宫内膜FST、Activin B表达水平  

Endometrial Follistatin and Activin expression levels during implantation period in patients with repeated implant failure

作　　者：李伟伟 刘聪 闫娅妮 殷秀荣 史凤颖 李丽玮 LI Weiwei;LIU Cong;YAN Yani;YIN Xiurong;SHI Fengying;LI Liwei(Department of Reproductive Medicine,Qinhuangdao Municipal Maternal and Child Health Care Hospital,Qinhuangdao,Hebei 066000,China;Department of Obstetrics,Qinhuangdao Municipal Maternal and Child Health Care Hospital,Qinhuangdao,Hebei 066000,China)

机构地区：[1]河北省秦皇岛市妇幼保健院生殖医学科,066000 [2]河北省秦皇岛市妇幼保健院产科,066000

出　　处：《重庆医学》2021年第9期1518-1521,1525,共5页Chongqing medicine

基　　金：河北省科技攻关计划(182777165);2019年度河北省医学科学研究课题计划(20191390)。

摘　　要：目的探讨卵泡抑素(FST)、活化素B(Activin B)在反复种植失败患者种植期子宫内膜的表达情况及其影响。方法选取2018年5月至2019年12月在该院生殖医学科进行体外受精与胚胎移植(IVF-ET)或卵泡浆内单精子显微注射(ICSI)治疗的不孕患者,由于男方因素或者输卵管因素于该院首次进行辅助生殖技术(ART)助孕,其中成功妊娠的患者作为对照组;至少进行3次移植(新鲜或者解冻的胚胎,每次至少有1枚优质胚胎,移植优质胚胎数大于或等于4)均未妊娠的患者作为反复植入失败(RIF)组;每组15例。采用免疫发光化学法检测两组患者血中性激素和抗缪勒管激素(AMH)的水平;采用超声监测移植日内膜的厚度;采用Western blot检测两组患者内膜组织中FST、Activin B、骨形态蛋白4(BMP4)、Smad3蛋白的表达情况。结果两组患者性激素、AMH及移植日内膜厚度比较,差异均无统计学意义(P>0.05)。RIF组患者FST的表达水平明显低于对照组,而Activin B的表达水平明显高于对照组,差异均有统计学意义(P<0.05)。RIF组患者内膜中BMP4的表达明显低于对照组,而Smad3水平明显高于对照组,差异均有统计学意义(P<0.05)。结论FST和Activin B在反复种植失败患者的种植窗期的子宫内膜中起重要的调节作用。Objective To investigate the expression situation of follistatin(FST)and Activin B in the endometrium of the patients with repeated implantation failure during implantation.Methods The infertile patients treated by IVF-ET or ICSI in the hospital from May 2018 to December 2019 were selected.Among them,the patients with successful pregnancy after the first assisted reproductive technology(ART)in this hospital due to the male factors or fallopian tube factors served as the control group;the patients without pregnancy by conducting at least 3 times of transfers(fresh or thawed embryos,at least one high-quality embryo each time,and the number of high-quality embryos transferred≥4)served as the repeated implantation failure(RIF)group;there were 15 cases in each group.The levels of blood hormones and antimullerian hormone(AMH)were detected by adopting the immunoluminescence chemical method;the thickness of the endometrium on the day of transplantation was monitored by ultrasound;the expression of FST,Activin B,bone morphogenetic protein 4(BMP4)and Smad3 protein in the two groups were detected by Western blot.Results The sex hormones,AMH and thickness of the endometrium on the day of transplantation had no statistical dfference between the two groups(P>0.05).The expression level of FST in the RIF group was significantly lower than that of the control group,while the expression level of Activin B was significantly higher than that of the control group,and the differences were statistically significant(P<0.05).The expression of BMP4 in the RIF group was significantly lower than that in the control group,while the Smad3 level was significantly higher than that in the control group,and the differences were statistically significant(P<0.05).Conclusion FST and Activin B play an important regulatory role in the endometrium during the implantation window period in the patients with repeated implantation failure.

关 键 词：反复植入失败 卵泡抑素 活化素 子宫内膜 辅助生殖技术 

分 类 号：R711.6[医药卫生—妇产科学]