艾灸与针刺对慢性萎缩性胃炎大鼠胃黏膜细胞凋亡及NF-κB、Bcl-2基因表达的影响  

Effect of moxibustion and acupuncture on gastric mucosal cell apoptosis and expression of NF-κB, Bcl-2 in chronic atrophic gastritis rats

作　　者：周巍 杨青[1] 舒文娜 黎铭玉 周兰 常小荣[1] 钟欢[1] 刘密 ZHOU Wei;YANG Qing;SHU Wen-na;LI Ming-yu;ZHOU Lan;CHANG Xiao-rong;ZHONG Huan;LIU Mi(School of Acupuncture and Massage,Hunan University of Chinese Medicine,Changsha 410208,China;Liuyang Hospital of Traditional Chinese Medicine,Liuyang 410300,Hunan Province)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208 [2]浏阳市中医医院,湖南浏阳410300

出　　处：《针刺研究》2021年第4期284-288,共5页Acupuncture Research

基　　金：国家重点基础研究发展计划(973项目,No.2015CB554502);国家自然科学基金项目(No.81574082、81904097);湖南省科技人才托举工程项目(No.2019TJ-Q04);湖南省“芙蓉学者奖励计划”(湘教通[2020]58号);湖南省高层次卫生人才“225”工程培养项目(湘卫函[2019]196号);湖南省121创新人才培养工程(湘人社函[2019]192号)。

摘　　要：目的:通过观察慢性萎缩性胃炎(chronic atrophic gastritis, CAG)大鼠胃黏膜细胞凋亡水平及核转录因子-κB(NF-κB)、B淋巴细胞瘤-2(Bcl-2)基因表达变化来探究针灸对CAG的治疗机制。方法:将健康雄性SD大鼠随机分为正常组、模型组、艾灸组、针刺组,每组12只。采用1-甲基-3-硝基-1-亚硝基胍(MNNG)联合多因素法(MNNG+乙醇灌胃+饥饱失常法)造模12周制备CAG大鼠模型。艾灸组予"足三里""中脘"悬灸15 min,针刺组予"足三里""中脘"直刺15 min,均每日1次,连续治疗2周。HE染色法观察各组大鼠胃黏膜形态变化,TUNEL法测定并计算各组大鼠胃黏膜细胞凋亡指数,实时荧光定量PCR法检测各组大鼠胃黏膜组织NF-κB、Bcl-2的基因表达水平。结果:模型组大鼠肉眼下胃黏膜色白淡暗,皱襞低平且有显著出血点,光镜下黏膜固有层腺体萎缩,排列错乱且数量显著减少,炎性细胞浸润;艾灸组和针刺组胃黏膜形态均得到改善。与正常组比较,模型组胃黏膜细胞凋亡指数显著增加(P<0.01);与模型组比较,艾灸组与针刺组胃黏膜细胞凋亡指数均显著降低(P<0.01)。与正常组比较,模型组胃黏膜组织NF-κB、Bcl-2基因表达显著升高(P<0.01);与模型组比较,艾灸组、针刺组胃黏膜组织NF-κB、Bcl-2基因表达均显著降低(P<0.05,P<0.01);与艾灸组比较,针刺组胃黏膜组织Bcl-2基因表达明显降低(P<0.01)。结论:传统针灸疗法可以下调CAG大鼠胃黏膜细胞凋亡指数,平衡胃黏膜细胞过度凋亡,降低NF-κB、Bcl-2基因表达,缓解大鼠胃黏膜炎性反应,保护胃黏膜组织,针灸调控胃黏膜细胞凋亡的机制可能与降低NF-κB、Bcl-2基因表达有关。Objective To observe the effect of moxibustion and acupuncture on apoptosis in gastric mucosal cell and expression of NF-κB, Bcl-2 in chronic atrophic gastritis(CAG) rats, so as to explore its mechanisms underlying improvement of CAG. Methods Forty-eight male SD rats were randomly divided into normal, model, moxibustion and acupuncture groups, with 12 rats in each group. The CAG model was established by gavage of N-methyl-N’-nitro-N-nitroso-guanidine(MNNG) solution and irregular diet for 12 weeks. Moxibustion or acupuncture was applied to "Zusanli"(ST36) and "Zhongwan"(CV12) for 15 min, once daily for two weeks in the moxibustion or acupuncture group. The histopathological changes of the gastric mucosa were observed by HE staining. The apoptosis index of gastric mucosa was measured by TUNEL method. The gene expression levels of NF-κB and Bcl-2 were detected by real-time PCR. Results After modeling, the color of gastric mucosa was pale and dark, with low folds and significant bleeding points. The glands in lamina propria were atrophied, arranged disorderly, and the numbers were significantly reduced, with inflammatory cells infiltrated. Those histopathological changes were evidently milder in the moxibustion and acupuncture groups. Compared with the normal group, the apoptosis index, NF-κB and Bcl-2 gene expression of the model group were significantly increased(P<0.01). After the treatment, the apoptosis index, NF-κB and Bcl-2 gene expression were significantly reduced in the moxibustion and acupuncture groups(P<0.05,P<0.01). The NF-κB expression was lower in the acupuncture group than that of moxibustion group(P<0.01). Conclusion Moxibustion and acupuncture therapy can improve the injury of gastric mucosa in CAG rats, which may be associated with its function in down-regulating the expression of NF-κB and Bcl-2 genes in the gastric mucosa.

关 键 词：慢性萎缩性胃炎 针灸 细胞凋亡 核转录因子-ΚB B淋巴细胞瘤-2 

分 类 号：R245.3[医药卫生—针灸推拿学]