栀子不同生育期根际土壤细菌群落结构的动态变化  

Variation of Bacterial Community Structure in Gardenia jasminoides Rhizosphere at Different Growth Stages

作　　者：李巧玲 肖忠[1] 任明波[1] 韩凤[1,2] 胡开治 LI Qiao-ling;XIAO Zhong;REN Ming-bo;HAN Feng;HU Kai-zhi(The Research Institute of Medicine Plantation of Chongqing,Nanchuan 408435,China;Chongqing Engineering Research Center for Genuine Medicinal Material Standard Production,Nanchuan 408435,China)

机构地区：[1]重庆市药物种植研究所,重庆南川408435 [2]重庆市道地药材规范化生产工程技术研究中心,重庆南川408435

出　　处：《土壤通报》2021年第2期346-354,共9页Chinese Journal of Soil Science

基　　金：国家重点研发计划项目(2017YFC1700704);重庆市科研院所绩效激励专项项目(cstc2018jxjl-jbky80002)资助。

摘　　要：以三年生栀子(Gardenia jasminoides Ellis)的根际土壤为研究对象,分析了栀子不同生育期根际细菌群落结构的动态变化,以期为栀子的高产栽培提供科学依据。高通量测序结果显示,栀子各生育期土壤细菌多样性呈先升高后降低的趋势,在果实膨大期多样性最丰富;且每个生育期根际细菌群落的优势菌属不同,花蕾期的优势菌属为芽单孢菌属(Gemmatimonas),盛花期为cSubgroup6,果实膨大期为藓杆菌属(Bryobacter),盛果期为Burkholderia-Caballeronia-Paraburkholderia。此外,通过UPGMA聚类分析可将四组样品聚为两类,花蕾期和盛花期聚为一类,果实膨大期与盛果期聚为另一类。同时,冗余分析(RDA)结果表明:不同土壤环境因子对栀子根际细菌群落组成的影响不同,芽单孢菌属、CandidatusSolibacter、鞘氨醇单胞菌属(Sphingomonas)及藓杆菌属的相对丰度与土壤pH、碱解氮、速效钾含量及酸性磷酸酶、酸性蛋白酶活性呈显著正相关。综合分析表明,栀子根际土壤养分、酶活性及根际细菌组成均随生育期而发生波动,生产中可根据各指标变化规律进行有效调控,为栀子高产栽培提供参考。The variation of bacterial community composition in the rhizosphere soil of three-year-old Gardenia jasminoides at different growth stages was analyzed,aiming to provide a scientific basis for the cultivation of high-yield Gardenia J.The results of high-throughput sequencing showed that the bacterial community structure in the rhizosphere soil of Gardenia J.was different among growth periods.The dominant bacterial genus at the flower bud period was Gemmatimonas,was cSubgroup6 at the flowering period,but it was Bryobacter at the fruit expansion period and Burkholderia-Caballeronia-Paraburkholderia in the fruiting period.The UPGMA cluster analysis showed that the bacterial community structure in the rhizosphere soil was similar between the flower bud stage and the full bloom stage,while it was similar between the fruit expansion stage and the full fruit stage.RDA analysis results showed that different soil environmental factors had different effects on the composition of bacterial community in the rhizosphere soil.The relative abundances of Gemmatimonadaceae,CandidatusSolibacter,Sphingomonas and Bryobacter were significantly and positively correlated with pH,alkali-hydrolyzale nitrogen,available potassium,acid phosphatase and acid protease.Consequently,nutrients,enzyme activities,and bacteria community composition in the rhizosphere soil was dynamically changed with growth periods.

关 键 词：栀子 高通量测序 生育期 根际细菌 土壤微生物 

分 类 号：S147.2[农业科学—肥料学]