小麦山农4143抗黑胚病遗传分析及抗性遗传位点检测  

Genetic analysis and detection of resistance loci for black point in wheat genotype Shannong4143

作　　者：王丝雨 李巧云[1] 姜玉梅[1] 徐凯歌 李梦钰 牛吉山[1] 闫亚洲 WANG Si-yu;LI Qiao-yun;JIANG Yu-mei;XU kai-ge;LI Meng-yu;NIU Ji-shan;YAN Ya-zhou(National Engineering Research Centre for Wheat,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学/国家小麦工程技术研究中心,郑州450046

出　　处：《植物病理学报》2021年第2期225-234,共10页Acta Phytopathologica Sinica

基　　金：国家重点研发计划项目(2018YFD0300707);河南省科技攻关项目(202102110021)。

摘　　要：黑胚病是小麦生产的重要籽粒病害,麦根腐平脐蠕孢(Bipolaris sorokiniana)是黑胚病的主要致病菌。为分析小麦抗黑胚病遗传规律并检测抗性位点,本研究以抗黑胚病小麦品系山农4143与感病品系宛原白1号的F7代重组自交系(RIL)群体为材料,于2018~2019年在3个试验点种植,采用"孢子液喷洒、套袋保湿"的方法进行接菌鉴定,用"主基因+多基因混合遗传模型"进行遗传分析,并挑选极端抗、感自交系构建混池、结合小麦660K SNP芯片进行混合分组分析(BSA)。研究结果显示小麦品系山农4143对黑胚病抗性符合"4对主基因加性-上位性遗传模型",主基因遗传力为0.88~0.95;通过BSA检测到黑胚病抗性位点36个,分别位于1A、2B(6)、2D(2)、3A、3B(2)、3D、4A(6)、4D、5A(4)、5B(6)、5D(3)、7A、7B和7D共14条染色体上,A、B、D染色组上分别为13、 15和8个,36个位点中有18个为抗黑胚病新鉴定位点。本研究结果为小麦抗黑胚病位点的精细定位和抗性育种中分子标记的开发奠定了基础。Black point disease, caused by the dominant pathogen Bipolaris sorokiniana, is a severe wheat grain disease. In order to analyze the genetic characters and detect the resistance loci for black point, the resistant genotype Shannong4143 and susceptible genotype Wanyuanbai1 and their recombinant inbred line(RIL) population at F7 were planted at three experimental locations in 2018-2019 wheat growing season. Resistance identification was carried out using the method of spraying spore suspension of B. sorokiniana and bagging for moisture. The genetic analysis was carried out by mixed inheritance model of major genes plus polygenes. The extreme resistant and susceptible inbred lines were selected to build a mixed pool. Combining 660 K SNP arrays, the resistant loci were detected by bulked segregate analysis(BSA). The results showed that: the resistance of wheat to black point caused by B. sorokiniana was in accordance with the "additive-epistatic inheritance model of 4 major genes", and heritability of the major gene was 0.88-0.95. Thirty-six loci were detected by BSA, which were located on 14 chromosomes of 1 A, 2 B(6), 2 D(2), 3 A, 3 B(2), 3 D, 4 A(6), 4 D, 5 A(4), 5 B(6), 5 D(3), 7 A, 7 B and 7 D, respectively. A total of 13, 15, and 8 loci were from the A, B, and D genomes, respectively. Among the 36 loci detected, 18 loci were new loci for resistance to black point. The results of this study laid a foundation for the fine mapping of resistance loci to black point and development of molecular markers in the breeding for resistant cultivars to black point.

关 键 词：小麦 黑胚病 麦根腐平脐蠕孢 遗传分析 抗性位点 

分 类 号：S432.44[农业科学—植物病理学]