河北省梨树褐斑病菌对不同机制杀菌剂的敏感性及其与戊唑醇敏感性的关系  

Sensitivity of Septoria piricola Desm from pear in Hebei Province to different classes of fungicides and correlation coefficient with tebuconazole

作　　者：毕秋艳[1] 赵建江[1] 张诗琪 韩秀英[1] 吴杰 路粉[1] 王文桥[1] Bi Qiu-yan;Zhao Jian-jiang;Zhang Shi-qi;Han Xiu-ying;Wu Jie;Lu Fen;Wang Wen-qiao(Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Integrated Pest Management Center of Hebei Province,Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,Hebei,China)

机构地区：[1]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,农业部华北北部作物有害生物综合治理重点实验室,保定071000

出　　处：《植物病理学报》2021年第2期248-257,共10页Acta Phytopathologica Sinica

基　　金：国家重点研发计划(2016YFD0200505-6)。

摘　　要：为明确河北省梨树褐斑病菌(Septoria piricola Desm)对戊唑醇和双胍三辛烷基苯磺酸盐、吡唑醚菌酯、环丙唑醇等不同作用机制杀菌剂的敏感性及交互抗性,采用室内菌丝生长速率法和田间小区试验分别测定了不同杀菌剂对11个地市分离到的133株病菌菌株的毒力,以及对梨树褐斑病的防治效果,研究了病菌对戊唑醇与对双胍三辛烷基苯磺酸盐、吡唑醚菌酯、环丙唑醇等药剂敏感性的相关性。结果显示:戊唑醇对梨树褐斑病菌供试133株菌株的EC_(50)范围在0.0392~2.0522μg·mL^(-1)之间,平均EC_(50)为0.5486±0.0012μg·mL^(-1);不同县市内的菌株对戊唑醇EC_(50)的最大值和最小值之比为2.7~7.4;各县市的菌株对戊唑醇敏感性差异不显著,戊唑醇EC_(50)平均值变化范围在0.1639~0.9124μg·mL^(-1)之间。将梨树褐斑病菌对双胍三辛烷基苯磺酸盐和吡唑醚菌酯EC_(50)平均值0.2621±0.0014μg·mL^(-1)、0.2750±0.0017μg·mL^(-1)分别作为该病菌对上述2种杀菌剂的敏感基线;梨褐斑病菌对戊唑醇的敏感性与其对双胍三辛烷基苯磺酸盐、吡唑醚菌酯的敏感性之间无相关性,与其对环丙唑醇的敏感性存在相关性。戊唑醇与双胍三辛烷基苯磺酸盐、戊唑醇与吡唑醚菌酯可以复配使用,最佳增效配比分别为1∶6和4∶1。田间防病试验结果表明:戊唑醇与双胍三辛烷基苯磺酸盐(30+180μg·mL^(-1))、戊唑醇与吡唑醚菌酯(100+25μg·mL^(-1))田间防效与单剂之间差异显著,1次用药后10 d防效分别为95.49%、93.48%,3次用药后10 d防效分别为86.51%、87.40%,3次用药后50 d防效分别为69.02%、70.33%,持效性均明显优于单剂。Resistance detecting and monitoring are critical to manage the fungicides and pathogen populations. We collected 133 isolates of Septoria piricola Desm from pear from 11 counties in Hebei Province. The sensitivities to tebuconazole, iminoctadine trialbesilate, pyraclostrobin and cyproconazole were determined by the mycelial growth method in the laboratory. The correlation between tebuconazole and iminoctadine trialbesilate, pyraclostrobin and cyproconazole were further evaluated. The results showed that EC_(50) values of tebuconazole for all isolates ranged from 0.0392 to 2.0522 μg·mL^(-1) with the average at 0.5486±0.0012 μg·mL^(-1). The ratio of lowest and highest EC_(50) values of tebuconazole for the isolates from the different regions ranged from 2.7 to 7.4. All the isolates did not exhibit the significant difference on the sensitivity to tebuconazole with the average EC_(50) values from 0.1639 to 0.9124 μg·mL^(-1). In compare with the baseline sensitivities for mycelia growth of S. piricola to iminoctadine trialbesilate and pyraclostrobin with the average values of 0.2621±0.0014 μg·mL^(-1) and 0.2750±0.0017 μg·mL^(-1), respectively, there was no significant correlation of sensitivity between tebuconazole and iminoctadine trialbesilate, pyraclostrobin, while correlation to cyproconazole. Therefore, tebuconazole and iminoctadine trialbesilate, tebuconazole and pyraclostrobin can be mixed, and the best mix ratio is 1∶6 and 4∶1, respectively. The control efficacy, compared with the single fungicide application for pear brown spot caused by S. piricola, was significant differences between tebuconazole + iminoctadine trialbesilate(30+180 μg·mL^(-1)) and tebuconazole + pyraclostrobin(100+25 μg·mL^(-1)) that was 95.49%, 93.48% in 10 days after spraying application just once, and 86.51%, 87.40% or 69.02%, 70.33% in 10 or 50 days after the third applications, respectively. The combination of the fungicides displayed a significantly better effectiveness and duration of protection than with their u

关 键 词：河北省 梨树褐斑病菌 戊唑醇 不同机制杀菌剂 敏感性基线 

分 类 号：S432.44[农业科学—植物病理学]