玉米BTB家族基因的鉴定与表达规律分析  

Identification and expression analysis of BTB family genes in Zea mays

作　　者：刘鹏飞[1] 张康 李玉琦[1] 周帆[1] 白华[1] 藏金萍[1] 曹宏哲 邢继红[1] 董金皋[1] LIU Peng-fei;ZHANG Kang;LI Yu-qi;ZHOU Fan;BAI Hua;ZANG Jin-ping;CAO Hong-zhe;XING Ji-hong;DONG Jin-gao(Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北省植物生理与分子病理学重点实验室/河北农业大学真菌毒素与植物分子病理学实验室,保定071000

出　　处：《植物病理学报》2021年第2期268-281,共14页Acta Phytopathologica Sinica

基　　金：粮食丰产增效科技创新专项(2016YFD0300704);国家玉米产业技术体系(CARS-02-12)。

摘　　要：本研究利用生物信息学方法对玉米BTB家族基因进行系统发育、保守结构域、组织特异性以及在玉米抵抗生物和非生物胁迫过程中的表达规律进行分析。发现玉米70个BTB家族蛋白除AC206223.3外均含有保守的BTB结构域,可分为11个亚家族。玉米BTB家族基因具有明显的组织表达特性,在高温、低温、紫外、高盐和干旱胁迫下以及拟轮枝镰孢(Fusarium verticillioide)侵染过程中的表达水平呈现出明显的差异。BTB-TAZ亚家族基因在水杨酸、茉莉酸、乙烯处理后表达水平发生明显的变化。结果表明BTB家族基因在玉米抵抗生物和非生物胁迫中发挥重要的作用,为阐明玉米BTB家族基因的功能及其机制奠定了基础。In this study, bioinformatics methods were used to conduct phylogenetic, conservative domain, tissue-specific, and expression regularity analysis of BTB family genes during resistance to biological and abiotic stresses in Zea mays. It was found that all 70 BTB family proteins in Z. mays contain a conserved BTB domain except AC206223.3, which can be divided into 11 subfamilies. The BTB family genes of maize had obvious tissue expression characteristics, and the expression levels show obvious differences under high temperature, low temperature, ultraviolet, high salt, drought stress, and in the process of Fusarium verticillioide infection. The expression levels of BTB-TAZ subfamily genes were significantly changed after SA, JA and ET treatment. These results showed that BTB family genes play an important role in resisting biological and abiotic stresses. It would lay the foundation for elucidating the function and mechanism of BTB family genes in Z. mays.

关 键 词：玉米 BTB/POZ家族 生物胁迫 非生物胁迫 表达分析 

分 类 号：S432.1[农业科学—植物病理学]