引起河北省保定市白术根腐病的病原镰刀菌种类鉴定  

Identification of pathogenic Fusarium species causing Atractylodes macrocephala root rot in Baoding, Hebei Province

作　　者：纪莉景[1] 肖颖[1] 李耀发[1] 贾海民[1] 王亚娇[1] 吴玉星 孔令晓[1] JI Li-jing;XIAO Ying;LI Yao-fa;JIA Hai-min;WANG Ya-jiao;WU Yu-xing;KONG Ling-xiao(Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Integrated Pest Management Center of Hebei Province,Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,China)

机构地区：[1]河北省农林科学院植物保护研究所,农业部华北北部作物有害生物综合治理重点实验室,河北省有害生物综合防治工程技术研究中心,保定071000

出　　处：《植物病理学报》2021年第2期282-286,共5页Acta Phytopathologica Sinica

基　　金：河北省财政项目(2019-1-1-3)。

摘　　要：白术(Atractylodes macrocephala Koidz.)是菊科苍术属多年生草本植物,具重要的药用价值和经济效益,而其根腐病在各产区发生普遍。河北省保定地区为白术秧苗的生产基地,一年生白术种植地块根腐病的病株率在5%~20%,二年生地块的病株率达到30%~50%,造成严重减产。In order to identify the pathogenic fungi of Atractylodes macrocephala root rot in Baoding, Hebei Province, eighty strains of Fusarium species were isolated from 120 A. macrocephala plants with root rot symptoms with the isolation frequency at 66.7%. Fusarium species were identified by molecular techniques with specific primers, TEF-1α gene sequencing together with morphological characteristics. Thirty-nine F. solani strains were identified with the highest percentage at 48.8%, and 32 F. oxysporum strains were isolated with the percentage at 40.0%. Seven F. equiseti and two F. brachygibbosum strains accounted for 8.8% and 2.5%, respectively. The pathogenicity test on A. macrocephala showed that F. solani and F. oxysporum caused the typical symptoms of root rot. Koch’s postulates were fulfilled following re-isolation and identification of the isolates of F. solani and F. oxysporum. Taken together, the results showed that F. solani and F. oxysporum were the pathogens of A. macrocephala root rot in Baoding, Hebei Province.

关 键 词：种植地块 多年生草本植物 种类鉴定 根腐病 河北省保定市 苍术属 镰刀菌 病株率 

分 类 号：S432.44[农业科学—植物病理学]