药物流产出血模型小鼠雌、孕激素水平与Th17/Treg亚群的关系  

The relationship between estrogen and progesterone levels and Th17/Treg subgroups in the hemorrhagic model mice after medical abortion

作　　者：胡晓燕 郭桂芝 肖婷婷 HU Xiao-yan;GUO Gui-zhi;XIAO Ting-ting(Emergency Obstetrics and Gynecology Department of the Affiliated Hospital of Guizhou Medical University,Guizhou 550004,China;Pharmaceutical Science of Guizhou Medical University)

机构地区：[1]贵州医科大学附属医院急诊妇产科,550004 [2]贵州医科大学药剂学教研室

出　　处：《天津医药》2021年第5期490-494,共5页Tianjin Medical Journal

摘　　要：目的研究药物流产出血模型小鼠的雌、孕激素水平与Th17/Treg亚群的关系。方法选取清洁级Balb/c雌性小鼠32只,通过随机数字表法分为正常妊娠组(A组)、小剂量米非司酮组(B组)、大剂量米非司酮组(C组)、米非司酮+宫清颗粒组(D组),每组8只。B、C、D组小鼠于妊娠第6天时分别使用米非司酮0.88 mg·kg^(-1)·d^(-1)、1.5 mg·kg^(-1)·d^(-1)及米非司酮(1.5 mg·kg^(-1)·d^(-1))^(+)宫清颗粒组(308.16 mg·kg^(-1)·d^(-1))灌胃。采用改良碱性正铁血红素法检测子宫出血量;采用放射免疫法检测血清雌二醇(E2)、孕酮(P)水平;采用流式细胞术双染色法检测子宫组织Th17(CD3^(+)CD4^(+)IL-17A^(+))、Treg(CD4^(+)CD25^(+)Foxp3^(+))及Th17/Treg亚群比值;采用逆转录聚合酶链式反应(RT-PCR)法检测子宫组织白细胞介素(IL)-17A、IL-10、转化生长因子(TGF)-βmRNA水平。结果B、C、D组子宫出血量依次减少。药物流产后,A、B、C、D组小鼠E2水平依次升高,而P水平依次降低。Th17亚群依次升高,Treg亚群依次降低,Th17/Treg亚群比值依次升高(均P<0.05)。IL-17A mRNA相对表达量依次升高,而IL-10、TGF-βmRNA相对表达量依次降低(均P<0.05)。血清E2与Th17、Th17/Treg和IL-17A水平呈正相关,与Treg、IL-10和TGF-β水平呈负相关;血清P与Treg、IL-10和TGF-β水平呈正相关,与Th17、Th17/Treg和IL-17A水平呈负相关(均P<0.05)。结论药物流产后的异常子宫出血血清E2、P水平变化与Th17/Treg亚群比值变化关系密切,诱导Th17/Treg亚群向Th17偏移有助于减少子宫出血。Objective To study the relationship between estrogen(E2)and progesterone(P)levels and Th17/Treg subgroups in the hemorrhagic model mice after medical abortion.Methods Thirty-two female Balb/c mice with clean grade were selected and divided into normal pregnancy group(group A),low-dose mifepristone group(group B),high-dose mifepristone group(group C)and mifepristone+gongqing granules group(group D)by random number table method,with 8 mice in each group.Mice in group B,C and D were gavaged with mifepristone 0.88 mg·kg^(-1)·d^(-1),1.5 mg·kg^(-1)·d^(-1) and mifepristone(1.5 mg·kg^(-1)·d^(-1))+gongqinggranules(308.16 mg·kg^(-1)·d^(-1))during d 6 pregnancy,respectively.Alkaline hematin photometric method was used to detect uterine blood loss.The serum E2 and P levels were detected by rdioimmunoassay(RIA).The uterus tissue Th17(CD3^(+)CD4^(+)IL-17A^(+)),Treg(CD4^(+)CD25^(+)Foxp3^(+))and the ratio of Th17/Treg subsets were detected by flow cytometry double staining method,and the uterus tissue interleukin 17A(IL-17A),interleukin 10(IL-10)and transforming growth factor(TGF-β)mRNA levels were detected by RT-PCR method.Results The uterine bleeding volume decreased in turn in group B,group C and group D(P<0.05).After drug abortion,the E2 levels increased in turn in group A,group B,group C and group D,while the P levels decreased in turn in group A,group B,group C and group D(P<0.05).The ratio of Th17 and Th17/Treg subgroups of mice decreased in turn in the four groups,while the Treg subgroup ratio decreased in turn(P<0.05).The levels of IL-17A mRNA increased in turn in the four groups(P<0.05),and the levels of IL-10 mRNA and TGF-βmRNA were decreased in turn in the four groups(P<0.05).The serum level of E2 was positively correlated with Th17,Th17/Treg and IL-17A mRNA,and negatively correlated with Treg,IL-10 and TGF-β(all P<0.05).The serum level of P was positively correlated with Treg,IL-10 and TGF-β,and negatively correlated with Th17,Th17/Treg and IL-17A(all P<0.05).Conclusion The changes of serum E2 and P leve

关 键 词：流产 人工 子宫出血 雌二醇 孕酮 TH17细胞 T淋巴细胞 调节性 

分 类 号：R719.31[医药卫生—妇产科学]