植物CRISPR/Cas技术研究进展及其在林业科学研究中的应用  

Current research progress of plant CRISPR/Cas technology and its application in forestry science

作　　者：胡凯强 廖家凯 席飞虎 高鹏飞 陈凯 魏文桃 丁家治 苗苗 顾连峰 HU Kaiqiang;LIAO Jiakai;XI Feihu;GAO Pengfei;CHEN Kai;WEI Wentao;DING Jiazhi;MIAO Miao;GU Lianfeng(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2021年第3期289-300,共12页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省林木种苗科技攻关六期项目(201904);福建省科技创新团队项目(118/KLA18069A)。

摘　　要：近年来,CRISPR/Cas系统在基因编辑领域展现出强大的定点编辑能力,在植物基础研究和分子育种等方面发挥了不可替代的作用.本文综述了CRISPR/Cas系统的发展历程,及其在植物研究应用中的遗传转化方法和鉴定方法等;同时还介绍了这种基因编辑技术在植物领域的多种研究新方向;并详细概述了CRISPR/Cas系统在林木基因功能分析鉴定等基础科研方面的应用情况;最后展望了该技术在林业科学研究中的应用前景,以期为后续相关的林木遗传改良等研究提供参考.In recent years,CRISPR/Cas technology springs up in the field of gene editing,exhibiting great site-specific editing capability.It plays an irreplaceable role in the basic research of plants and molecular breeding.This article systematically reviews the development history of the CRISPR/Cas system,and methods for genetic transformation and target sites identification in plant research.Several new research directions of this gene editing technology in plant science are summarized.Particularly,the application of CRISPR/Cas system in the basic research of forestry such as function analysis and identification of genes from tree is elaborated.Finally,challenges and future application prospects of this technology in forestry scientific research are discussed,providing insights into the subsequent research on the genetic improvement of forest.

关 键 词：基因编辑 林木 分子育种 遗传转化 

分 类 号：Q78[生物学—分子生物学]