稻瘟病菌MoSCJ1基因的生物学功能分析  

Biological function of MoSCJ1 in Magnaporthe oryzae

作　　者：陈浩[1] 王敏[1] 杨子峰 廖煌 吴欢 凌菡 汤蔚 CHEN Hao;WANG Min;YANG Zifeng;LIAO Huang;WU Huan;LING Han;TANG Wei(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学闽台作物有害生物生态防控国家重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2021年第3期309-316,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建农林大学科技创新专项基金项目(CXZX2019007G,CXZX2020014A)。

摘　　要：以稻瘟病菌野生型菌株Guy11为材料,利用基因敲除和回补得到稻瘟病菌的突变体ΔMoscj1和互补菌株ΔMoscj1/MoSCJ1,并以野生型为对照,分析了MoSCJ1的生物学功能.结果发现:突变体ΔMoscj1在基本培养基上的生长速率显著下降,同时,MoSCJ1基因的缺失导致突变体对细胞壁胁迫剂更加敏感;但MoSCJ1基因的缺失并不影响稻瘟病菌的无性繁殖、附着胞发育及致病性.这说明MoSCJ1参与调控稻瘟病菌的营养生长和对细胞壁胁迫剂的应答过程,不参与调控该菌的无性繁殖及致病过程.We obtained the MoSCJ1 gene deletion mutant from the wild type Guy11 of Magnaporthe oryzae and the complemented strain via gene knock-out and complementation technologies,and then analyzed the biological functions of MoSCJ1 in rice blast fungus.We found that the deletion of MoSCJ1 significantly impaired the vegetative growth of M.oryzae on basic medium and led to higher sensitivity to cell wall stressors.However,the loss of MoSCJ1 had no effects on the sporulation,appressorium development and pathogenicity.In conclusion,MoSCJ1 is likely to be involved in the regulation of vegetative growth and cell wall integrity of M.oryzae,instead of the regulation of asexual propagation and pathogenicity.

关 键 词：稻瘟病菌 MoSCJ1 营养生长 细胞壁完整性 

分 类 号：S432.44[农业科学—植物病理学]